中国现代医学杂志
中國現代醫學雜誌
중국현대의학잡지
CHINA JOURNAL OF MODERN MEDICINE
2005年
1期
21-23,27
,共4页
一氧化氮%骨折%骨折愈合
一氧化氮%骨摺%骨摺愈閤
일양화담%골절%골절유합
nitric oxide%fracture%fracture healing
目的探讨一氧化氮在骨折愈合中的可能作用.方法Wistar大鼠120只随机分为三组:L-精氨酸组,L-硝基-精氨酸甲基脂(L-NAME)组及对照组,建立股骨闭合性骨折模型,骨折后3 d,1,2和4周分别取每组10只大鼠骨折处骨痂行大体及HE染色观察.结果大体所见,在骨折后不同时期L-NAME组骨折处骨痂生成少,骨痴塑形慢,L-精氨酸组与其正好相反.L-NAME有使骨折愈合延迟的趋势,但与对照组比较并没有显著的意义(P=0.10);L-精氨酸组大鼠无论在骨痂塑形和骨折愈合程度上都强于L-NAME组(P<0.05).结论在骨折愈合过程中加入L-NAME使骨折愈合减慢,而加入L-精氨酸会促进骨折愈合.一氧化氮可能促进骨折的愈合过程.
目的探討一氧化氮在骨摺愈閤中的可能作用.方法Wistar大鼠120隻隨機分為三組:L-精氨痠組,L-硝基-精氨痠甲基脂(L-NAME)組及對照組,建立股骨閉閤性骨摺模型,骨摺後3 d,1,2和4週分彆取每組10隻大鼠骨摺處骨痂行大體及HE染色觀察.結果大體所見,在骨摺後不同時期L-NAME組骨摺處骨痂生成少,骨癡塑形慢,L-精氨痠組與其正好相反.L-NAME有使骨摺愈閤延遲的趨勢,但與對照組比較併沒有顯著的意義(P=0.10);L-精氨痠組大鼠無論在骨痂塑形和骨摺愈閤程度上都彊于L-NAME組(P<0.05).結論在骨摺愈閤過程中加入L-NAME使骨摺愈閤減慢,而加入L-精氨痠會促進骨摺愈閤.一氧化氮可能促進骨摺的愈閤過程.
목적탐토일양화담재골절유합중적가능작용.방법Wistar대서120지수궤분위삼조:L-정안산조,L-초기-정안산갑기지(L-NAME)조급대조조,건립고골폐합성골절모형,골절후3 d,1,2화4주분별취매조10지대서골절처골가행대체급HE염색관찰.결과대체소견,재골절후불동시기L-NAME조골절처골가생성소,골치소형만,L-정안산조여기정호상반.L-NAME유사골절유합연지적추세,단여대조조비교병몰유현저적의의(P=0.10);L-정안산조대서무론재골가소형화골절유합정도상도강우L-NAME조(P<0.05).결론재골절유합과정중가입L-NAME사골절유합감만,이가입L-정안산회촉진골절유합.일양화담가능촉진골절적유합과정.
[Objective] To evaluate the possible function of NO in fracture healing. [Methods] 120 Wistar rats were divided into 3 groups including L-arginine, L-NAME and the control group. The closed fractured model of each rat was built in the right femur. The callus of the fractured site was amputated and observed by the gross appearance and HE stain in the 3rd day, the first, second and fourth week after fracture. [Results] The gross observation of fracture in different stage indicated relatively small callus after the L-NAME intake. This was the same with the callus modelliing. Compared to these findings, completely opposite results were seen in the L-arginine group.When compared to the control group, the tendency of fracture healing was seen in the L-NAME group, but it had no significant difference (P =0.1). The degree of remodeling of the bone and fracture healing in the group of L-arginine was stronger than that in L-NAME (P <0.05). [Conclusion] L-NAME' s intake will inhibit the process after the fracture, which indicates NO may play an important role in the process of fracture healing.