CAJ | 학술논문

目的观察血管性痴呆大鼠海马CAI区神经元MAP-2、NF表达变化,探讨缺血再灌注损伤后神经元骨架蛋白表达变化与学习记忆的相互关系.方法采用Morris水迷宫筛选空间学习记忆能力正常的雄性Wistar大鼠60只;正常组(Norma1);椎动脉焊扎组(VO);双侧颈总动脉结扎20 nin再灌组(BCCO/R);全脑缺血20min再灌组(GI/R);后三组又分为存活7,14和30天组,处死前作水迷宫检测.用免疫组织化学方法检测MAP-2和NF表达,常规尼氏染色法镜下计数海马CAI区存活神经元.结果水迷宫检测GI/R组大鼠潜伏期增加,尼氏染色证实GI/R组锥体神经元减少(P＜0.01).与Normal组比较,BCCO/R7 d组海马CAI区辐射层MAP-2表达减弱,神经元突起中NF表达减少(P＜0.05);而BCCO/R14 d和3O d组胞浆MAP--2和NF表达均无变化(P＞0.05).GI/R组海马CAI区MAP-2、NF的表达在突起中几乎消失,而在锥体神经元核周有强表达,与各对照组比较判别有统计学意义(P＜0.05).结论MAP-2、NF在神经元突起中减少,而在胞体中聚集,是神经元可塑性的体现,在血管性痴呆动物模型中,可能对神经元有保护作用.
목적관찰혈관성치태대서해마CAI구신경원MAP-2、NF표체변화,탐토결혈재관주손상후신경원골가단백표체변화여학습기억적상호관계.방법채용Morris수미궁사선공간학습기억능력정상적웅성Wistar대서60지;정상조(Norma1);추동맥한찰조(VO);쌍측경총동맥결찰20 nin재관조(BCCO/R);전뇌결혈20min재관조(GI/R);후삼조우분위존활7,14화30천조,처사전작수미궁검측.용면역조직화학방법검측MAP-2화NF표체,상규니씨염색법경하계수해마CAI구존활신경원.결과수미궁검측GI/R조대서잠복기증가,니씨염색증실GI/R조추체신경원감소(P＜0.01).여Normal조비교,BCCO/R7 d조해마CAI구복사층MAP-2표체감약,신경원돌기중NF표체감소(P＜0.05);이BCCO/R14 d화3O d조포장MAP--2화NF표체균무변화(P＞0.05).GI/R조해마CAI구MAP-2、NF적표체재돌기중궤호소실,이재추체신경원핵주유강표체,여각대조조비교판별유통계학의의(P＜0.05).결론MAP-2、NF재신경원돌기중감소,이재포체중취집,시신경원가소성적체현,재혈관성치태동물모형중,가능대신경원유보호작용.
Objective:To investigate the expression of MAP-2 and NF in CA1 region of hippocampal neurons and its relation with spatial learning and memory in vascular dementia model(VD).Methods:60Wistar rats with normal learning and memory confirmed by Morris water maze were divided into 4 groups group(GI/R).According to animal surviving period,the last three groups were subdivided into three groups,7,14 and 30days.These rats were tested again in Morris water maze before being sacrificed.Expression of MAP-2 and NF were detected by immunohistochemistry and the intensity of immunostaining was measured by MedHPIAS pathologic imaging-analysis system.Coronal slices with Nissl's staining were used to count neurons in hippocampus CAI regions.Results:Compared with groups normal,VO and BCCO/R,escape latency of GI/R group was longer following elongation of reperfusion(P＜0.01).In Hippocampus CA1 regions the number of neurons was not reduced in groups VO and BCCO/R,but decreased in GI/R groups(P＜0.01),and had significant differences among the GI/R 7,14 and 30d groups(P＜0.01).Compared with normal group,expressions of MAP-2 and NF in Hippocampus CAI region obviously reduced in BCCO/R7d group(P＜0.05),but in groups BCCO/R 14 d and 30 d their expressions had no significant change(P＞0.05).In groups GI/R the neurites with immunostaining of MAP-2 and NF were not observed,but strong staining of these two proteins was present in perikaryon of the neurons(P＜0.O5).Conclusions:The expression pattern of MAP-2 and NF indicates that neuronal plasticity is the responding to injury.The finding that MAP-2 and NF were aggregated in cytoplasm might imply their protective roles to neuron in vascular dementia.