中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
9期
215-217
,共3页
三叉神经%一氧化氮合酶%舌咽神经%迷走神经%神经元
三扠神經%一氧化氮閤酶%舌嚥神經%迷走神經%神經元
삼차신경%일양화담합매%설인신경%미주신경%신경원
背景:一氧化氮合酶(nitric oxide synthase,OS)广泛分布于中枢神经系统并调节神经活动,但鲜见在中枢神经脊上水平有关NOS参与内脏神经传入信息传递的报道.目的:探查三叉神经脊束间质核(interstitial nucleus of trigeminal tract.INV)内内脏传入终末与向臂旁核(parabrachial nucleus,BN)投射的NOS阳性神经元之间的联系.设计:以实验动物为研究对象,验证性对比观察.单位:一所军医大学解剖学教研室.材料:选择成年SD雄性大鼠10只进行动物试验.干预:分别给PBN及舌咽和迷走神经内注射四甲基罗丹明(tetramethvl rhodamine-dextran,MR),生物素化葡聚糖胺(biotinylated dextran-amine.BDA)并进行NOS免疫组织化学反应,结合激光扫描共聚焦显微镜观察.主要观察指标:观察逆行TMR标记细胞、NOS阳性细胞以及跨神经节BDA标记的传入终末在INV内的重叠分布和三者的相互关系.结果:逆行标记细胞主要位于注射侧的INV,大多属20μm以下的中、小型细胞.NOS阳性细胞与TMR逆行标记细胞分布区域重叠.计数显不:NOS/TMR双标记细胞分别占NOS阳性细胞总数的55%(17/31)和TMR逆行标记细胞总数的34%(17/49).跨神经节注射BDA标记的内脏神经初级传入终末点状膨体贴近双标记细胞胞体,呈紧密接触状.结论:存在经INV向PBN投射的内脏信息传导通路,作为神经递质和神经信息分子的一氧化氮可能参与其内脏伤害性信息的传递和调控.
揹景:一氧化氮閤酶(nitric oxide synthase,OS)廣汎分佈于中樞神經繫統併調節神經活動,但鮮見在中樞神經脊上水平有關NOS參與內髒神經傳入信息傳遞的報道.目的:探查三扠神經脊束間質覈(interstitial nucleus of trigeminal tract.INV)內內髒傳入終末與嚮臂徬覈(parabrachial nucleus,BN)投射的NOS暘性神經元之間的聯繫.設計:以實驗動物為研究對象,驗證性對比觀察.單位:一所軍醫大學解剖學教研室.材料:選擇成年SD雄性大鼠10隻進行動物試驗.榦預:分彆給PBN及舌嚥和迷走神經內註射四甲基囉丹明(tetramethvl rhodamine-dextran,MR),生物素化葡聚糖胺(biotinylated dextran-amine.BDA)併進行NOS免疫組織化學反應,結閤激光掃描共聚焦顯微鏡觀察.主要觀察指標:觀察逆行TMR標記細胞、NOS暘性細胞以及跨神經節BDA標記的傳入終末在INV內的重疊分佈和三者的相互關繫.結果:逆行標記細胞主要位于註射側的INV,大多屬20μm以下的中、小型細胞.NOS暘性細胞與TMR逆行標記細胞分佈區域重疊.計數顯不:NOS/TMR雙標記細胞分彆佔NOS暘性細胞總數的55%(17/31)和TMR逆行標記細胞總數的34%(17/49).跨神經節註射BDA標記的內髒神經初級傳入終末點狀膨體貼近雙標記細胞胞體,呈緊密接觸狀.結論:存在經INV嚮PBN投射的內髒信息傳導通路,作為神經遞質和神經信息分子的一氧化氮可能參與其內髒傷害性信息的傳遞和調控.
배경:일양화담합매(nitric oxide synthase,OS)엄범분포우중추신경계통병조절신경활동,단선견재중추신경척상수평유관NOS삼여내장신경전입신식전체적보도.목적:탐사삼차신경척속간질핵(interstitial nucleus of trigeminal tract.INV)내내장전입종말여향비방핵(parabrachial nucleus,BN)투사적NOS양성신경원지간적련계.설계:이실험동물위연구대상,험증성대비관찰.단위:일소군의대학해부학교연실.재료:선택성년SD웅성대서10지진행동물시험.간예:분별급PBN급설인화미주신경내주사사갑기라단명(tetramethvl rhodamine-dextran,MR),생물소화포취당알(biotinylated dextran-amine.BDA)병진행NOS면역조직화학반응,결합격광소묘공취초현미경관찰.주요관찰지표:관찰역행TMR표기세포、NOS양성세포이급과신경절BDA표기적전입종말재INV내적중첩분포화삼자적상호관계.결과:역행표기세포주요위우주사측적INV,대다속20μm이하적중、소형세포.NOS양성세포여TMR역행표기세포분포구역중첩.계수현불:NOS/TMR쌍표기세포분별점NOS양성세포총수적55%(17/31)화TMR역행표기세포총수적34%(17/49).과신경절주사BDA표기적내장신경초급전입종말점상팽체첩근쌍표기세포포체,정긴밀접촉상.결론:존재경INV향PBN투사적내장신식전도통로,작위신경체질화신경신식분자적일양화담가능삼여기내장상해성신식적전체화조공.
BACKGROUND: Nitric oxide synthase(NOS) is extensively distributed in the central nervous system and modulates neuronal activities. However,there are few reports about NOS' s involvement in nociceptive information transmission at the supraspinal level.OBJECTIVE: To investigate the relation between the primary afferent visceral terminials and the NOS-containing neurons which project from the interstitial nucleus of spinal trigeminal tract(INV) to the parabrachial nucleus(PBN).DESIGN: A controlled experimental observation based on the experimental animals.SETTING: Department of anatomy in a military medical university of Chinese PLA.MATERIALS: The experiment was conducted in the Department of Anatomy,Fourth Military Medical University of Chinese PLA,from January to December 2002. Ten adult male SD rats were selected in this experiment.INTERVENTIONS: Tetramethyl rhodamine-dextran(TMR) and biotinylated dextran-amine(BDA) were injected into the PBN and vago-glossopharyngeal nerves respectively combining with the immunohistochemical method for NOS and the observation with laser confocal scanning microscope.MAIN OUTCOME MEASUREES: Overlapping of TMR retrogradely labeled neurons,NOS positive labeled neurons and transganglionic tracing labeled terminals,and their relationship in INV.RESULTS: After injection of TMR into the PBN,the retrogradely labeled neurons,mostly small and medium-sized(below20 μm),were found in the INV ipsilateral to the injection side. The distribution of the NOS positive neurons overlapped with retrogradely labeled neurons in INV. The proportion of the number of double-labeled neurons with NOS/TMR was 55% (17/31)and 34% (17/49) in the whole population of the NOS positive neurons and retrogradely neurons,respectively. Some transganglionic tracing labeled terminals after BDA injection had close contact with the double-labeled neurons.CONCLUSION: The visceral nociceptive pathway exists from INV to PBN and NO may act as the transmitter or neural signal molecule in the transmission and regulation of visceral nociceptive process.