中国药物化学杂志
中國藥物化學雜誌
중국약물화학잡지
Chinese Journal of Medicinal Chemistry
2008年
6期
452-456
,共5页
杜芝芝%宋成芝%郁步竹%罗晓东
杜芝芝%宋成芝%鬱步竹%囉曉東
두지지%송성지%욱보죽%라효동
滑桃树%镰刀菌属%抗菌%tetramic acid
滑桃樹%鐮刀菌屬%抗菌%tetramic acid
활도수%렴도균속%항균%tetramic acid
Trewia nudiflora%Fusarium%antibacteria%tetramic acid
目的 对滑桃树内生真菌Fusamum sp.2TnP1-2的抗菌活性次生代谢产物进行研究.方法 采用硅胶柱色谱、Sephadex LH-20、制备性薄层色谱等对次生代谢产物进行分离纯化.根据理化性质和波谱分析进行结构鉴定.利用纸片扩散法对这些化合物的抗真菌及抗细菌活性进行测试.结果 从该菌株PDA培养基发酵产物中分离得到3个化合物,分别鉴定为:trichosetin(N-demethyl eqmsetln,1),latentin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6α-epoxy-24(R)-methylcholesta-7,22-dien-3β-ol(3).结论 3个化合物均为首次从该真菌的代谢物中分离得到.trichosetin和lateritin具有抗金黄色葡萄球菌活性.
目的 對滑桃樹內生真菌Fusamum sp.2TnP1-2的抗菌活性次生代謝產物進行研究.方法 採用硅膠柱色譜、Sephadex LH-20、製備性薄層色譜等對次生代謝產物進行分離純化.根據理化性質和波譜分析進行結構鑒定.利用紙片擴散法對這些化閤物的抗真菌及抗細菌活性進行測試.結果 從該菌株PDA培養基髮酵產物中分離得到3箇化閤物,分彆鑒定為:trichosetin(N-demethyl eqmsetln,1),latentin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6α-epoxy-24(R)-methylcholesta-7,22-dien-3β-ol(3).結論 3箇化閤物均為首次從該真菌的代謝物中分離得到.trichosetin和lateritin具有抗金黃色葡萄毬菌活性.
목적 대활도수내생진균Fusamum sp.2TnP1-2적항균활성차생대사산물진행연구.방법 채용규효주색보、Sephadex LH-20、제비성박층색보등대차생대사산물진행분리순화.근거이화성질화파보분석진행결구감정.이용지편확산법대저사화합물적항진균급항세균활성진행측시.결과 종해균주PDA배양기발효산물중분리득도3개화합물,분별감정위:trichosetin(N-demethyl eqmsetln,1),latentin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6α-epoxy-24(R)-methylcholesta-7,22-dien-3β-ol(3).결론 3개화합물균위수차종해진균적대사물중분리득도.trichosetin화lateritin구유항금황색포도구균활성.
Aim To study antifungal and antibacterial secondary metabolites of Fusarium sp.2TnP1-2,an endophytic fungus isolated from Trewia nudiflora.Methods PDA fementation ex-tract was isolated by bio-assay guided fractionation and different column chromatography methods including silica gel column,Sephadex LH-20 column and preparative thin layer chromatography.Structures of these compounds were identified on the basis of spectroscopic analysis of 1D,2D-NMR,MS and comparison of chemical and physical data with authentic samples rePOrted in litera-tures.The antibacterial and antifungal activities of the isolated compounds were measured using the disc diffusion method.Results Three compounds were isolated and their structures were deter-mined to be trichosetin(N-demethyl equisetin,1),lateritin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6α-epoxy-24(R)-methylcholesta-7,22-di-en-3β-ol(3).Conclusion All the compounds were obtained from Fusarium for the first time.Tri-chosetin and lateritin possess antibacterial activity against Staphylococcus aureus.
