CAJ | 학술논문

研究了一种用T型微通道制备固体脂质纳米粒(solid lipid nanoparticles,SLN)的新方法.以softisan100(C_(10)～C_(18)的混合脂)丙酮溶液作为脂相,以Poloxamer 188水溶液为水相,用注射泵分别将脂相和水相注入T型通道的主道和支道内,两相在交叉口接触后形成明显的相界面,并继续沿主道向前流动.脂相中丙酮通过相界面迅速向水相扩散,随着流体的向前运动,脂相中脂的浓度不断增大至过饱和而形成固体脂质纳米粒(SLN).实验考察了两相流速和微通道尺寸对SLN粒径大小和粒径分布的影响.结果表明:在实验条件下,制得的SLN粒径在110～350 nm之间,多分散性指数小于0.24;T型通道交叉口的流场分布受两相相对流速的影响,并直接影响成粒规律,在不出现两相返混条件下,保持水相流速不变,SLN粒径随脂相流速增大而增大;保持脂相流速不变,粒径随水相流速的增大略有增大;通道尺寸越小所制得粒径也越小.
연구료일충용T형미통도제비고체지질납미립(solid lipid nanoparticles,SLN)적신방법.이softisan100(C_(10)～C_(18)적혼합지)병동용액작위지상,이Poloxamer 188수용액위수상,용주사빙분별장지상화수상주입T형통도적주도화지도내,량상재교차구접촉후형성명현적상계면,병계속연주도향전류동.지상중병동통과상계면신속향수상확산,수착류체적향전운동,지상중지적농도불단증대지과포화이형성고체지질납미립(SLN).실험고찰료량상류속화미통도척촌대SLN립경대소화립경분포적영향.결과표명:재실험조건하,제득적SLN립경재110～350 nm지간,다분산성지수소우0.24;T형통도교차구적류장분포수량상상대류속적영향,병직접영향성립규률,재불출현량상반혼조건하,보지수상류속불변,SLN립경수지상류속증대이증대;보지지상류속불변,립경수수상류속적증대략유증대;통도척촌월소소제득립경야월소.
A new method of preparing solid lipid nanoparticles (SLN) by using T-shaped junction microchannel was proposed. The lipid solution formed by dissolving Softisan 100 (triglyceride mixture of fatty acids with chain lengths of C_(10) to C_(18)) into acetone was used as lipid phase, and the surfactant solution formed by dissolving Poloxamer 188 into super pure water was used as aqueous phase; then they were injected into the main channel and branch channel of the T-shape junction microchannel, separately, by precision syringe pumps. The two phases injected meet at the junction of the T-shaped microchannel and form interface of phases. The solvent acetone in the lipid phase diffuses quickly into the aqueous phase through the interface, which causes the supersaturation of lipid and the formation of SLN. The effects of various operation conditions, such as various flow velocities of both lipid and aqueous phases and different dimensions of the microchannel, on the size and the polydispersity of the formed SLN were investigated. Under our experimental conditions, SLN with small size of 110～350 nm and narrow size distribution (polydispersity less than 0.24) were prepared successfully. The experimental results show that the flow field distribution at the T-junction is affected by the flow rates of the two phases, which also affect the diameter and size distribution of the prepared SLN obviously. Under the condition of not occurring backmixing of the two injected phases, the size of the prepared SLN increases with the increase of the aqueous phase velocity when the lipid phase velocity keeps unchanged, while it increases slightly with the increase of the lipid phase velocity when the aqueous phase velocity keeps unchanged. It was also found that the prepared SLN with smaller particle size could be obtained when a T-shaped microchannel with narrower main channel was used.