CAJ | 학술논문

采用微生物分离纯化方法,从制革厂含铬污泥中筛选分离Cr (Ⅵ)抗性菌株,并研究菌株对Cr(Ⅵ)的去除能力.共分离得到对50 mg/L Cr(Ⅵ)去除率大于50%的菌株20株,16S rRNA基因测序结果表明这些细菌主要属于Acinetobacter、 Microbacterium、Leucobacter、Ochrobactrum和Brachymonas属.对其中7株细菌,考察了菌株生长期、pH值和Cr(Ⅵ)浓度对菌株去除Cr(Ⅵ)效果的影响,结果表明,细胞在有较高代谢活性的条件下具较高的Cr(Ⅵ)去除能力;pH值对菌株去除Cr(Ⅵ)的能力具有显著影响,在50 mg/L Cr(Ⅵ)、pH值为7～8的条件下,Microbacterium属16号和21号菌株在36 h时对Cr (Ⅵ)的去除率达80%～95%;高浓度的Cr(Ⅵ)抑制菌株对Cr(Ⅵ)的去除能力,其中21号菌株在110 mg/L的Cr(Ⅵ)浓度下去除效果最佳,Cr(Ⅵ)去除率达80%.
채용미생물분리순화방법,종제혁엄함락오니중사선분리Cr (Ⅵ)항성균주,병연구균주대Cr(Ⅵ)적거제능력.공분리득도대50 mg/L Cr(Ⅵ)거제솔대우50%적균주20주,16S rRNA기인측서결과표명저사세균주요속우Acinetobacter、 Microbacterium、Leucobacter、Ochrobactrum화Brachymonas속.대기중7주세균,고찰료균주생장기、pH치화Cr(Ⅵ)농도대균주거제Cr(Ⅵ)효과적영향,결과표명,세포재유교고대사활성적조건하구교고적Cr(Ⅵ)거제능력;pH치대균주거제Cr(Ⅵ)적능력구유현저영향,재50 mg/L Cr(Ⅵ)、pH치위7～8적조건하,Microbacterium속16호화21호균주재36 h시대Cr (Ⅵ)적거제솔체80%～95%;고농도적Cr(Ⅵ)억제균주대Cr(Ⅵ)적거제능력,기중21호균주재110 mg/L적Cr(Ⅵ)농도하거제효과최가,Cr(Ⅵ)거제솔체80%.
Bacteria strains with Cr(Ⅵ) tolerant capacity were isolated from chromium-contaminated tannery sludge and the Cr (Ⅵ) removal characteristics of these isolates were studied. Twenty strains with the Cr (Ⅵ) removal efficiency greater than 50% under the condition of 50 mg/L Cr(Ⅵ) were obtained. These strains were closely related to species in the genera Acinetobacter; Microbacteri-um, Leucobacter, Ochrobactrum and Brachymonas by their 16S rRNA gene sequences. The effects of growth phase, pH and Cr (Ⅵ) concentration on the Cr (Ⅵ) reducing capacity for seven isolates were investigated. Cells with high activity showed higher Cr (Ⅵ) removal efficiency. pH affected the Cr (Ⅵ) removal capacity of these strains remarkably. The Cr (Ⅵ) removal efficiencies of strain No.16 and No.21, closely related to the species in the genus Microbacterium, were over 80% at the pH of 7-8 under the condition of 50 mg/L of Cr (Ⅵ). High concentration of Cr (Ⅵ) repressed the Cr (Ⅵ) removal capacity. However, strain No. 21 showed a removal efficiency of about 80% when the initial Cr (Ⅵ) concentration was 110 mg/L.