CAJ | 학술논문

哈尔滨市鲜肉中单核细胞增生性李斯特菌的分离鉴定及耐药性分析
합이빈시선육중단핵세포증생성리사특균적분리감정급내약성분석
Isolation and identification of antibiotic resistance of Listeria monocytogenes isolated from meat in Harbin

万方数据

中国预防兽医学报中國預防獸醫學報 중국예방수의학보
CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE
2009年 12期 929-932 ,共4页

尹录%杜艳芬%赫明雷%司微%刘慧芳%王春来%彭玮%王聃%李继昌%邵美丽%刘思国

윤록%두염분%혁명뢰%사미%류혜방%왕춘래%팽위%왕담%리계창%소미려%류사국

哈尔滨%鲜肉%单核细胞增生性李斯特茵%分离鉴定%耐药性哈爾濱%鮮肉%單覈細胞增生性李斯特茵%分離鑒定%耐藥性
합이빈%선육%단핵세포증생성리사특인%분리감정%내약성
Harbin%fresh meat%Listeria monocytogenes%isolation and characterization%drug resistanceCorresponding author

为了解哈尔滨市单核细胞增生性李斯特茵(Lm)的污染状况及耐药状况.在哈尔滨市市场随机采集158份鲜肉样品,采用显色培养基分离,API试剂条和PCR鉴定等方法对样品中的Lm进行分离鉴定,并通过Kirby-Barer法测定分离菌株对24种抗生素的耐药性.结果从鲜肉中共分离到Lm 23株,检出率为14.56%,其中鲜猪肉检出率最高,达20.00%(14/70);23株分离菌株中耐药菌株为22株,耐药率高达95.65%.这表明哈尔滨市鲜肉中存在一定程度的Lm污染,并且分离菌株存在较严重的耐药现象.应加强控制动物饲料亚治疗抗生素的使用并严格遵守休药期,防止耐药菌株产生进而控制食源性疾病的发生.
위료해합이빈시단핵세포증생성리사특인(Lm)적오염상황급내약상황.재합이빈시시장수궤채집158빈선육양품,채용현색배양기분리,API시제조화PCR감정등방법대양품중적Lm진행분리감정,병통과Kirby-Barer법측정분리균주대24충항생소적내약성.결과종선육중공분리도Lm 23주,검출솔위14.56%,기중선저육검출솔최고,체20.00%(14/70);23주분리균주중내약균주위22주,내약솔고체95.65%.저표명합이빈시선육중존재일정정도적Lm오염,병차분리균주존재교엄중적내약현상.응가강공제동물사료아치료항생소적사용병엄격준수휴약기,방지내약균주산생진이공제식원성질병적발생.
To investigate the contamination and drug resistance of Listeria monocytogenes in meat, 158 fresh meat samples were randomly collected from meat markets in Harbin, and 23 Listeria monocytogenes strains (14.6 % positive) were isolated and characterized by Coloration medium, API reagent strip and PCR. The contamination in raw pork is the highest, with 20.00 % (14/70) detected positive. The drug resistance of isolated strains was tested against antibacterial drugs by Kirby-Barer method, and all but one (95.65 %) displayed drug resistance. The results showed the Listeria monocytogenes contamination in fresh meat was evident in Harbin and drug resistant rate was very serious, In order to reduce the drug resistance pathogens it is necessary to control and reduce the use of antibiotics in animal feedstuff's.