CAJ | 학술논문

以抗枯萎病节瓜品种杂文20号为材料,利用抑制差减杂交(Suppression subtractive hybridiza-tion,SSH)技术,构建了枯萎菌(Fusarium oxysporum f. sp.beniacasa)诱导的节瓜SSH-cDNA文库.文库质量检测表明,差减杂交效率较高,质量较好.得到正向差减文库的滴度为3.64×10~5/CFU/mL,反向差减文库滴度为6.80×10~5 CFU/mL;正向和反向差减文库的重组率均大于90%,插入片段在400～800bp之间.
이항고위병절과품충잡문20호위재료,이용억제차감잡교(Suppression subtractive hybridiza-tion,SSH)기술,구건료고위균(Fusarium oxysporum f. sp.beniacasa)유도적절과SSH-cDNA문고.문고질량검측표명,차감잡교효솔교고,질량교호.득도정향차감문고적적도위3.64×10~5/CFU/mL,반향차감문고적도위6.80×10~5 CFU/mL;정향화반향차감문고적중조솔균대우90%,삽입편단재400～800bp지간.
By a suppression subtractive hybridization technique, a suppression subtractive hybridization cDNA library was constructed with resistant Chieh-qua cultivar Zajiao 20 leaf induced by Fusarium oxysporum f. sp. beniacasa. Detection and analysis of the library construction showed that the technique was efficient in the subtractive hybridization and a good qualitySSH-cDNA library was obtained. The titers of forward-and reverse-SSH-cDNA libraries were 3.64 × 10~5 CFU/mL and 6.80 ×10~5 CFU/mL, respectively. More than 90% clones were recombinant and the length of the insert fragments were ranging from400-800 bp in the forward-and reverse-SSH libraries.