CAJ | 학술논문

研究‘秦美'猕猴桃冷藏期外果肉、内果肉和中轴胎座3个组织区的光合色素、叶绿体细胞学及其光合放氧的变化特征.结果表明:‘秦美'猕猴桃果实各组织区光合色素的组分随着果实的冷藏处于动态变化之中;在冷藏各时期叶绿素(Chl)和(或)脱植基叶绿索的存在使果实始终呈现绿色;同一组织区Chl a,Chl b和Chl(a+b)含量随着冷藏时间延长而逐渐减少,Chl a/b则上升;在同一冷藏时期,各组织区Chl a,Chl b和Chl(a+b)含量高低为外果肉>内果肉>中轴胎座.冷藏开始,在显微镜明场下各组织区叶绿体均呈不规则球形,在荧光显微镜下则呈蜂窝状,一些淀粉粒从叶绿体中散落出来;随着冷藏时间延长,各组织区叶绿体数量减少,体积变小,淀粉粒水解,片层结构崩解;冷藏30天时,叶绿体淀粉粒数量明显变少,常见片层结构残迹.随着冷藏时间延长,各组织区光合放氧速率均下降,同一时期各冷藏时间光合放氧速率为外果肉>内果肉>中轴胎座.表明在冷藏过程中,猕猴桃各组织区的光合色素、叶绿体形态结构和光系统Ⅱ光合放氧是有差异的.
연구‘진미'미후도랭장기외과육、내과육화중축태좌3개조직구적광합색소、협록체세포학급기광합방양적변화특정.결과표명:‘진미'미후도과실각조직구광합색소적조분수착과실적랭장처우동태변화지중;재랭장각시기협록소(Chl)화(혹)탈식기협록색적존재사과실시종정현록색;동일조직구Chl a,Chl b화Chl(a+b)함량수착랭장시간연장이축점감소,Chl a/b칙상승;재동일랭장시기,각조직구Chl a,Chl b화Chl(a+b)함량고저위외과육>내과육>중축태좌.랭장개시,재현미경명장하각조직구협록체균정불규칙구형,재형광현미경하칙정봉와상,일사정분립종협록체중산락출래;수착랭장시간연장,각조직구협록체수량감소,체적변소,정분립수해,편층결구붕해;랭장30천시,협록체정분립수량명현변소,상견편층결구잔적.수착랭장시간연장,각조직구광합방양속솔균하강,동일시기각랭장시간광합방양속솔위외과육>내과육>중축태좌.표명재랭장과정중,미후도각조직구적광합색소、협록체형태결구화광계통Ⅱ광합방양시유차이적.
The variation characteristics of photosynthetic pigments, chloroplast cytology and its photosynthetic oxygen evolution rate were investigated in outer pericarp (OP) , inner pericarp (IP) and axile placenta (AP) of Actinidia deliciosa 'Qinmei' fruit during cold storage, obtain basic knowledge of quality keeping in process of cold storage.The results showed that the composition of photosynthetic pigments in each tissue zone changed with the time of refriferation.The presence of Chl and (or) chlide make the pulp assume green color; however with the fruit cold-stored time, Chl a, Chl b and Chl(a + b) contents decreased gradually and Chl a/b increased.At a given cold-stored stage, the orders of content of Chl a, Chl b and Chl (a + b) of each tissue zone of the fruit were OP > IP > AP.The chloroplasts were solid irregular sphericity under lightfield and honeycomb like under fluorescence microscope before cold storage.Some starch grains were spilled into the cytoplasm from the chloroplasts.The number and volume of the chloroplast of every tissue zone became less and smaller, starch grains hydrolyzed and chloroplasts disintegrated with cold storage.The starch grain became less significantly and remnant lamella structure was frequently found after 30 d cold storage.The photosynthetic oxygen evolution rates of each tissue zone decreased with cold storage and the orders of the rates were :OP > IP > AP at the same period of cold storage.It was concluded that there were differences among photosynthetic pigments, morphology structure of chloroplast and photosynthetic oxygen evolution rate in each tissue zone during cold storage.