国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2012年
2期
86-88
,共3页
陈派镇%林创兴%林广裕%马廉%肖泽曦%吴扬%冯学永%周晓华%邱晓燕
陳派鎮%林創興%林廣裕%馬廉%肖澤晞%吳颺%馮學永%週曉華%邱曉燕
진파진%림창흥%림엄유%마렴%초택희%오양%풍학영%주효화%구효연
手足口病%咽拭子%疱疹液%病原学
手足口病%嚥拭子%皰疹液%病原學
수족구병%인식자%포진액%병원학
Hand,foot and mouth disease%Throat swabs%Vesicle fluid%Etiology
目的 了解粤东地区2010年手足口病患儿的病原学特点并探讨实时RT-PCR法检测手足口病患儿咽拭子和疱疹液样本病原体的敏感性.方法 收集94例手足口病(重症35例,轻症59例)患儿的咽拭子和疱疹液样本,以肠道病毒(EV)通用型、柯萨奇病毒A16(CA16)型、肠道病毒71(EV71)型核酸检测试剂盒,应用实时RT-PCR法检测样本中的EV,并结合临床资料对样本阳性率的样本进行比较.结果 94份咽拭子样本中,EV通用型阳性59例(62.77％),EV71阳性49例(52.13％),CA16阳性18例(19.15％),其中35份重症患儿咽拭子样本中EV通用型阳性31例(88.57％),EV71阳性23例(65.71％),CA16阳性7例(20.00％)；94份疱疹液样本中EV通用型阳性44例(46.81％),EV71阳性33例(35.11％),CA16阳性15例(15.96％),其中35份重症患儿疱疹液样本中EV通用型阳性22例(62.86％),EV71阳性15例(42.86％),CA16阳性6例(17.14％).统计资料显示,咽拭子的阳性率高于疱疹液,EV71的检出率差异有统计学意义(x2=5.537,P＜0.05),重症患儿EV71阳性率高于轻症患儿,两组患儿的EV通用型及EV71检出差异有统计学意义(x2=15.889、4.125,P＜0.05).结论 2010年我院手足口病住院患儿的病原体主要为EV71和CA16,并且以EV71为主.应用实时RT-PCR法检测手足口病病原体,咽拭子的病毒检出率较疱疹液高,重症患儿的病毒检出率明显高于轻症患儿.
目的 瞭解粵東地區2010年手足口病患兒的病原學特點併探討實時RT-PCR法檢測手足口病患兒嚥拭子和皰疹液樣本病原體的敏感性.方法 收集94例手足口病(重癥35例,輕癥59例)患兒的嚥拭子和皰疹液樣本,以腸道病毒(EV)通用型、柯薩奇病毒A16(CA16)型、腸道病毒71(EV71)型覈痠檢測試劑盒,應用實時RT-PCR法檢測樣本中的EV,併結閤臨床資料對樣本暘性率的樣本進行比較.結果 94份嚥拭子樣本中,EV通用型暘性59例(62.77％),EV71暘性49例(52.13％),CA16暘性18例(19.15％),其中35份重癥患兒嚥拭子樣本中EV通用型暘性31例(88.57％),EV71暘性23例(65.71％),CA16暘性7例(20.00％)；94份皰疹液樣本中EV通用型暘性44例(46.81％),EV71暘性33例(35.11％),CA16暘性15例(15.96％),其中35份重癥患兒皰疹液樣本中EV通用型暘性22例(62.86％),EV71暘性15例(42.86％),CA16暘性6例(17.14％).統計資料顯示,嚥拭子的暘性率高于皰疹液,EV71的檢齣率差異有統計學意義(x2=5.537,P＜0.05),重癥患兒EV71暘性率高于輕癥患兒,兩組患兒的EV通用型及EV71檢齣差異有統計學意義(x2=15.889、4.125,P＜0.05).結論 2010年我院手足口病住院患兒的病原體主要為EV71和CA16,併且以EV71為主.應用實時RT-PCR法檢測手足口病病原體,嚥拭子的病毒檢齣率較皰疹液高,重癥患兒的病毒檢齣率明顯高于輕癥患兒.
목적 료해월동지구2010년수족구병환인적병원학특점병탐토실시RT-PCR법검측수족구병환인인식자화포진액양본병원체적민감성.방법 수집94례수족구병(중증35례,경증59례)환인적인식자화포진액양본,이장도병독(EV)통용형、가살기병독A16(CA16)형、장도병독71(EV71)형핵산검측시제합,응용실시RT-PCR법검측양본중적EV,병결합림상자료대양본양성솔적양본진행비교.결과 94빈인식자양본중,EV통용형양성59례(62.77％),EV71양성49례(52.13％),CA16양성18례(19.15％),기중35빈중증환인인식자양본중EV통용형양성31례(88.57％),EV71양성23례(65.71％),CA16양성7례(20.00％)；94빈포진액양본중EV통용형양성44례(46.81％),EV71양성33례(35.11％),CA16양성15례(15.96％),기중35빈중증환인포진액양본중EV통용형양성22례(62.86％),EV71양성15례(42.86％),CA16양성6례(17.14％).통계자료현시,인식자적양성솔고우포진액,EV71적검출솔차이유통계학의의(x2=5.537,P＜0.05),중증환인EV71양성솔고우경증환인,량조환인적EV통용형급EV71검출차이유통계학의의(x2=15.889、4.125,P＜0.05).결론 2010년아원수족구병주원환인적병원체주요위EV71화CA16,병차이EV71위주.응용실시RT-PCR법검측수족구병병원체,인식자적병독검출솔교포진액고,중증환인적병독검출솔명현고우경증환인.
Objective To investigate the etiologic characteristics of hand,foot and mouth disease(HFMD)in eastern Guangdong and to compare the sensibility of RT-PCR testing pathogen in throat swabs and vesicle fluid.Methods Throat swabs and vesicle fluid were collected from 94 children with HFMD(including 35 severe cases and 59 mild cases).Nucleic acids of three type of viruses of enterovirus coxsackievirus A16(CA16),enterovirus 71(EV 71 )and EV were detected by RT-PCR.Results In all the 94 cases,the EV,EV71 and CA16 positive rate in throat swabs samples were 62.77％ (59 cases),52.13％ (49 cases) and 19.15％ (18 cases) respectively,including 88.57％ (31 cases),65.71％(23 cases) and 20.00％(7 cases) positive rates in 35 severe cases.However,EV,EV71 and CA16 positive rate in 94 vesicle fluid were 46.81％ (44 cases),35.11％ ( 33 cases),15.96％ ( 15 cases),including 62.86％ (22cases),42.86％(15 cases) and 17.14％(6 cases) in 35 severe cases.The positive rate of EV71 by RT-PCR in throat swabs was higher than in vesicle fluid ( x2 =5.537,P ＜ 0.05),and the positive rates of EV and EV71 in severe cases were also much higher (x2 =15.889,4.125,P ＜ 0.05).Conclusions EV71 and CA16 are common in children in our hospital,and most patients are infected with EV71.RT-PCR is hypersensitive for testing HFMD pathogens in throat swabs.The positive rate of HFMD in severe case is much higher than mild cases.
