CAJ | 학술논문

目的观察丙型肝炎病毒NS3蛋白对QSG7701细胞survivin的影响及其与细胞凋亡的关系,以探讨HCV NS3蛋白是否通过调节survivin的表达来参与对细胞凋亡的调控,进而参与HCV相关性肝癌的发生.方法采用Western blot、免疫细胞化学检测血清饥饿前后细胞survivin蛋白表达的变化,采用TUNEL法检测血清饥饿前后细胞凋亡的变化.结果 HCV NS3蛋白促进QSG7701细胞survivin的表达,血清饥饿诱导凋亡后,survivin表达降低,HCV NS3蛋白亦表现出促进survivin表达的作用,效果呈浓度依赖性和时间依赖性.经血清饥饿诱导凋亡后,HCV NS3蛋白抑制凋亡.结论 HCV NS3蛋白可能通过促进QSG7701细胞survivin的表达从而抑制细胞凋亡,对HCV相关性肝癌的形成有一定作用.
목적 관찰병형간염병독NS3단백대QSG7701세포survivin적영향급기여세포조망적관계,이탐토HCV NS3단백시부통과조절survivin적표체래삼여대세포조망적조공,진이삼여HCV상관성간암적발생.방법 채용Western blot、면역세포화학검측혈청기아전후세포survivin단백표체적변화,채용TUNEL법검측혈청기아전후세포조망적변화.결과 HCV NS3단백촉진QSG7701세포survivin적표체,혈청기아유도조망후,survivin표체강저,HCV NS3단백역표현출촉진survivin표체적작용,효과정농도의뢰성화시간의뢰성.경혈청기아유도조망후,HCV NS3단백억제조망.결론 HCV NS3단백가능통과촉진QSG7701세포survivin적표체종이억제세포조망,대HCV상관성간암적형성유일정작용.
Objective To study the regulation of HCV NS3 protein on the expression of survivin prorein in QSG7701 cell and the interacion with apoptosis for investigating carcinogenesis mechanism of HCV NS3 protein in human liver cell. Methods Apoptosis induced by serum starvation or not, the expressions of survivin protein are detected by Western Blot and immunocytochemistry, the apoptosis indexes are examined by TUNEL.Results HCV NS3 protein activates the expression of survivin in QSG7701 cell. After apoptosis induced by serum starvation, the expressions of survivin are down-regulated. HCV NS3 protein also activates the expression of survivin which exhibits the tendency of time and concentration in QSG7701 cell. At the same time, HCV NS3 protein inhibits apoptosis induced by serum deprivation in QSG7701 cell. Conclusion HCV NS3 protein inhibits apoptosis by up-regulating the expression of survivin protein in QSG7701 cell, contributes to malignant transformation of hepatocytes and hepatocellular carcinogenesis related HCV.