CAJ | 학술논문

目的观察维生素K2联合磷脂酰胆碱抑制肝癌的协同作用并探讨其作用机制.方法采用细胞计数试剂盒(CCK-8)细胞增殖实验评价维生素K2(1.25 ～ 500.00 μmol/L)联合磷脂酰胆碱( 1.25 ～400.00 μmol/L)抑制人肝癌细胞SMMC-7721的浓度依赖性和时间依赖性(24、48、72 h),采用流式细胞仪检测肝癌细胞凋亡率,采用Western blot法检测半胱氨酸-天冬氨酸蛋白酶前体(procaspase)-3、procaspase-8和procaspase-9的活性表达.结果维生素K2(500.00 μmol/L)联合磷脂酰胆碱( 20.00 μmol/L)共同作用72 h可致使65.1％的肝癌细胞发生凋亡,实验组procaspase-3和procaspase-9表达差异有统计学意义(P＜0.05),procaspase-8表达差异无统计学意义(P＞0.05).结论维生素K2联合磷脂酰胆碱对抑制人肝癌细胞有协同作用,其机制可能依赖于Caspase-9的活化.
목적 관찰유생소K2연합린지선담감억제간암적협동작용병탐토기작용궤제.방법 채용세포계수시제합(CCK-8)세포증식실험평개유생소K2(1.25 ～ 500.00 μmol/L)연합린지선담감( 1.25 ～400.00 μmol/L)억제인간암세포SMMC-7721적농도의뢰성화시간의뢰성(24、48、72 h),채용류식세포의검측간암세포조망솔,채용Western blot법검측반광안산-천동안산단백매전체(procaspase)-3、procaspase-8화procaspase-9적활성표체.결과 유생소K2(500.00 μmol/L)연합린지선담감( 20.00 μmol/L)공동작용72 h가치사65.1％적간암세포발생조망,실험조procaspase-3화procaspase-9표체차이유통계학의의(P＜0.05),procaspase-8표체차이무통계학의의(P＞0.05).결론 유생소K2연합린지선담감대억제인간암세포유협동작용,기궤제가능의뢰우Caspase-9적활화.
Objective To investigate the synergistic effect and mechanism of vitamin K2 and phosphatidylcholine (PC) on hepatocellular carcinoma (HCC) cells.Methods Synergism between vitamin K2 ( 1.25-500.O0 μ moI/L) and PC ( 1.25-400.00 μmoI/L) was studied in human hepatoma cell line SMMC-7721 in vitro with cell counting Kit-8 ( CCK-8 ) kit and Annexin V-FITC/PI kit.The key proteins of Caspase apoptosis pathway were detected by using Western blotting.Results The combined administration of vitamin K2 (500.00 μmol/L) and PC (20.00 μmol/L) induced 65.1％ apoptosis rate of HCC cells at 72nd h.Western blotting revealed the significant difference in the differential expression of procaspase-3 and procaspase-9 in the experimental group ( P ＜ 0.05 ),while procaspase-8 changed unsignificantly ( P ＞0.05 ).Conclusion Vitamin K2 and PC exhibited synergistic effects against hepatocarcinogenesis via activating Caspase-9.