中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
5期
307-311
,共5页
程娟%冷曙光%李海山%牛勇%孙耀峰%段化伟%宾萍%张林媛%梁学邈%林函%王忠旭%戴宇飞%李斌%郑玉新
程娟%冷曙光%李海山%牛勇%孫耀峰%段化偉%賓萍%張林媛%樑學邈%林函%王忠旭%戴宇飛%李斌%鄭玉新
정연%랭서광%리해산%우용%손요봉%단화위%빈평%장림원%량학막%림함%왕충욱%대우비%리빈%정옥신
焦炭%诱变剂%博来霉素%彗星试验
焦炭%誘變劑%博來黴素%彗星試驗
초탄%유변제%박래매소%혜성시험
Coke%Mutagens%Bleomycin%Comet assay
目的 研究焦炉作业工人外周血淋巴细胞对诱变剂博来霉素(bleomycin,BLM)损伤的敏感性.方法 以94名焦炉作业工人(暴露组)和64名选矿工人(对照组)作为研究对象.收集班后尿,测定尿中1-羟基芘水平反映多环芳烃暴露内剂量.肘静脉血分离淋巴细胞,体外培养20 h后,用8 μg/ml BLM处理30 min,彗星试验评价细胞DNA损伤水平.BLM处理前后DNA损伤的差即为个体对诱变剂的敏感性.结果 两组研究对象在年龄、性别、吸烟饮酒状况等方面差异均无统计学意义.焦炉工尿中1-羟基芘水平(9.0 μg/L,95%CI:6.8～11.7)显著高于对照(1.5 μg/L,95%CI:1.3～1.7)(t=-9.317,P＜0.01).对照组诱变剂敏感性为14.9(95%CI:13.7～16.3),焦炉工为17.7(95%CI:16.3～19.3),差异有统计学意义(t=-2.583,P=0.01).进一步按焦炉工尿1-羟基芘水平几何均数(9.0 μg/L)分层,发现高1-羟基芘水平组焦炉工对BLM的敏感性显著高于低1-羟基芘水平组(F=4.001,P=0.05).对照人群中吸烟者对BLM造成的损伤更为敏感.未发现年龄、饮酒、焦炉作业工龄、外暴露等级等因素对BLM敏感性有显著影响.结论 焦炉逸散物的暴露能够增加外周血淋巴细胞对BLM损伤的敏感程度,可能是焦炉作业工人中肿瘤高发的一个原因.
目的 研究焦爐作業工人外週血淋巴細胞對誘變劑博來黴素(bleomycin,BLM)損傷的敏感性.方法 以94名焦爐作業工人(暴露組)和64名選礦工人(對照組)作為研究對象.收集班後尿,測定尿中1-羥基芘水平反映多環芳烴暴露內劑量.肘靜脈血分離淋巴細胞,體外培養20 h後,用8 μg/ml BLM處理30 min,彗星試驗評價細胞DNA損傷水平.BLM處理前後DNA損傷的差即為箇體對誘變劑的敏感性.結果 兩組研究對象在年齡、性彆、吸煙飲酒狀況等方麵差異均無統計學意義.焦爐工尿中1-羥基芘水平(9.0 μg/L,95%CI:6.8～11.7)顯著高于對照(1.5 μg/L,95%CI:1.3～1.7)(t=-9.317,P＜0.01).對照組誘變劑敏感性為14.9(95%CI:13.7～16.3),焦爐工為17.7(95%CI:16.3～19.3),差異有統計學意義(t=-2.583,P=0.01).進一步按焦爐工尿1-羥基芘水平幾何均數(9.0 μg/L)分層,髮現高1-羥基芘水平組焦爐工對BLM的敏感性顯著高于低1-羥基芘水平組(F=4.001,P=0.05).對照人群中吸煙者對BLM造成的損傷更為敏感.未髮現年齡、飲酒、焦爐作業工齡、外暴露等級等因素對BLM敏感性有顯著影響.結論 焦爐逸散物的暴露能夠增加外週血淋巴細胞對BLM損傷的敏感程度,可能是焦爐作業工人中腫瘤高髮的一箇原因.
목적 연구초로작업공인외주혈림파세포대유변제박래매소(bleomycin,BLM)손상적민감성.방법 이94명초로작업공인(폭로조)화64명선광공인(대조조)작위연구대상.수집반후뇨,측정뇨중1-간기비수평반영다배방경폭로내제량.주정맥혈분리림파세포,체외배양20 h후,용8 μg/ml BLM처리30 min,혜성시험평개세포DNA손상수평.BLM처리전후DNA손상적차즉위개체대유변제적민감성.결과 량조연구대상재년령、성별、흡연음주상황등방면차이균무통계학의의.초로공뇨중1-간기비수평(9.0 μg/L,95%CI:6.8～11.7)현저고우대조(1.5 μg/L,95%CI:1.3～1.7)(t=-9.317,P＜0.01).대조조유변제민감성위14.9(95%CI:13.7～16.3),초로공위17.7(95%CI:16.3～19.3),차이유통계학의의(t=-2.583,P=0.01).진일보안초로공뇨1-간기비수평궤하균수(9.0 μg/L)분층,발현고1-간기비수평조초로공대BLM적민감성현저고우저1-간기비수평조(F=4.001,P=0.05).대조인군중흡연자대BLM조성적손상경위민감.미발현년령、음주、초로작업공령、외폭로등급등인소대BLM민감성유현저영향.결론 초로일산물적폭로능구증가외주혈림파세포대BLM손상적민감정도,가능시초로작업공인중종류고발적일개원인.
Objective To investigate the sensitivity to bleomycin (BLM) in peripheral blood lymphocytes (PBL) among coke-oven workers. Methods Ninty-four coke-oven workers with exposure to a high level of polycyclic aromatic hydrocarbons and 64 non-coke-oven workers (control) were recruited into this study. PBL was challenged by 8 μg/ml BLM, a known carcinogen, to induce certain amount of DNA damage, the difference of olive tail moment (TM) measured by comet assay before and after BLM treatment reflected the sensitivity towards mutagens. Results The distribution of age, sex, and prevalence of smoking and drinking were not significantly different between these two groups. The geometric mean of urinary 1-hydroxypyrene (1-OHP) was significantly higher in coke-oven workers than in controls (9.0 versus 1.5 μg/L, t = - 9. 317, P ＜ 0. 01 ). The coke-oven workers showed significantly higher sensitivity to BLM than controls ( 17.7 versus 14. 9, t = - 2. 583, P = 0. 01 ). A large inter-group difference in sensitivity to BLM was observed in both controls and coke-oven workers. Stratification analysis revealed the significant association between high 1-OHP level ( ＞ 9. 0 μg/L) and increased sensitivity to BLM ( F = 4. 001, P =0. 05 ) among coke-oven workers. Smoking subjects showed a significant higher value of sensitivity than nonsmokers in controls but not in coke-oven workers. No significant difference was observed between age,drinking status, coking history or external exposure class and BLM sensitivity. Conclusion Exposure to coke oven emission could increase the sensitivity to mutagens, which might be a reason of high incidence of lung cancer among coke-oven workers.
