CAJ | 학술논문

目的观察骨形成蛋白-2(BMP-2)基因转染兔骨髓基质细胞(MSCs)复合藻酸钙修复兔膝关节软骨缺损的效果.方法取4周龄的新西兰兔骨髓细胞,体外培养得到MSCs.选用24只成年新西兰兔,在两侧股骨髁非负重区造成全层软骨缺损模型,A组(实验组)植入BMP-2转染的MSCs+藻酸钙,B组(对照组Ⅰ)植入空质粒转染的MSCs+藻酸钙,C组(对照组Ⅱ)植入不含MSCs的藻酸钙,D组(对照组Ⅲ)旷置.12周后,观察软骨缺损修复情况及组织学评价.结果实验组修复组织基本光滑,有大量Ⅱ型胶原蛋白表达,3个对照组修复组织Ⅱ型胶原蛋白表达量少,仍有明显缺损.实验组和3个对照组组织学评分差异有统计学意义(P＜0.05).结论 BMP-2转染兔MSCs修复软骨缺损效果优于其他对照组.
목적 관찰골형성단백-2(BMP-2)기인전염토골수기질세포(MSCs)복합조산개수복토슬관절연골결손적효과.방법 취4주령적신서란토골수세포,체외배양득도MSCs.선용24지성년신서란토,재량측고골과비부중구조성전층연골결손모형,A조(실험조)식입BMP-2전염적MSCs+조산개,B조(대조조Ⅰ)식입공질립전염적MSCs+조산개,C조(대조조Ⅱ)식입불함MSCs적조산개,D조(대조조Ⅲ)광치.12주후,관찰연골결손수복정황급조직학평개.결과 실험조수복조직기본광활,유대량Ⅱ형효원단백표체,3개대조조수복조직Ⅱ형효원단백표체량소,잉유명현결손.실험조화3개대조조조직학평분차이유통계학의의(P＜0.05).결론 BMP-2전염토MSCs수복연골결손효과우우기타대조조.
Objective Rabbit bone marrow stromal cells (MSCs) transfected by bone morphogenetic protein-2 (BMP-2) gene mixed with calciumalginate to repair defects of articular cartilage of rabbit knee,then to observe the result of repairing.Methods Extract bone marrow cells from a four-week-old New Zealand rabbit.Isolated and purified bone marrow stromal cells in vitro.Choose 24 grown-up New Zealand rabbits and divide them into 4 groups randomly.Full-thickness articular cartilage defects were made in the non-weight-bearing area of the bilateral femoral condyles in New Zealand rabbits.Transfer the different four groups complex component to the different defect place of the articular cartilage:In group A,MSCs + calciumalginate complex,which has been transfected by BMP-2 (Experimental Group) ; In group B,MSCs + calciumalginate complex,which transfected by Empty plasmid (Control GroupⅠ) ; In group C,calciumalginate without cells (Control GroupⅡ) ; In group D,without any treatment (Control GroupⅢ).The femoral condyles were harvested at the end of 12 weeks,and then observe the condition of repairation of cartilage defects and make assessments of histology.Results Repair Tissue part in experimental group was almost flat smoothly,consisting of a large of type-Ⅱ collagen.In 3 control groups,the type-Ⅱ collagen of Repair Tissue was bit and had apparent defection.There were significant difference between the experimental group and the three control groups in histological evaluation (P ＜ 0.05).Conclusion The group which was transfered with BMP-2 + MSCs is superior to control groups in the effect of repairing cartilage defects.