中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2011年
17期
23-25
,共3页
宁寒冰%王艳红%张连峰%李继昌
寧寒冰%王豔紅%張連峰%李繼昌
저한빙%왕염홍%장련봉%리계창
TRF2%Rap1%胃癌%多药耐药
TRF2%Rap1%胃癌%多藥耐藥
TRF2%Rap1%위암%다약내약
TRF2%Rap1%Gastric cancer%Multidrug resistance
目的 应用RNA干扰技术抑制多药耐药衍生胃癌细胞系TRF2表达,观察其对Rap1表达的影响.方法 根据pSilencer3.1 - H1载体要求构建TRF2 siRNA真核表达载体,分别转染SGC7901/ADR和SGC7901/VCR 细胞,免疫荧光技术和Western blot方法检测Rap1表达.结果 成功构建TRF2 siRNA真核表达载体能显著抑制耐药细胞TRF2表达,siRNA转染细胞中Rap1表达明显降低.结论 TRF2调控Rap1表达.
目的 應用RNA榦擾技術抑製多藥耐藥衍生胃癌細胞繫TRF2錶達,觀察其對Rap1錶達的影響.方法 根據pSilencer3.1 - H1載體要求構建TRF2 siRNA真覈錶達載體,分彆轉染SGC7901/ADR和SGC7901/VCR 細胞,免疫熒光技術和Western blot方法檢測Rap1錶達.結果 成功構建TRF2 siRNA真覈錶達載體能顯著抑製耐藥細胞TRF2錶達,siRNA轉染細胞中Rap1錶達明顯降低.結論 TRF2調控Rap1錶達.
목적 응용RNA간우기술억제다약내약연생위암세포계TRF2표체,관찰기대Rap1표체적영향.방법 근거pSilencer3.1 - H1재체요구구건TRF2 siRNA진핵표체재체,분별전염SGC7901/ADR화SGC7901/VCR 세포,면역형광기술화Western blot방법검측Rap1표체.결과 성공구건TRF2 siRNA진핵표체재체능현저억제내약세포TRF2표체,siRNA전염세포중Rap1표체명현강저.결론 TRF2조공Rap1표체.
Objective To evaluate the effect of TRF2 siRNA on Rap1 expression in multidrug -resistant gastric cancer cells.Methods TRF2 siRNA eukaryotic expression vector based on pSilencer 3.1 -H1 vector was constructed and transfected into SGC7901/ADR and SGC7901/VCR cells respectively ; the expression of Rap1 were detected by immunofluorescence and Western blot.Results The expression of both TRF2 and Rap1 was significantly inhibited by TRF2 siRNA in multidrug - resistant gastric cancer cells.Conclusions TRF2 regulate the expression of Rap1.
