遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2000年
10期
902-910
,共9页
范敏%许勇%张海英%任华中%康国斌%王永健%陈杭
範敏%許勇%張海英%任華中%康國斌%王永健%陳杭
범민%허용%장해영%임화중%강국빈%왕영건%진항
西瓜%分子标记%遗传图谱%数量性状位点(QTL)%果实性状
西瓜%分子標記%遺傳圖譜%數量性狀位點(QTL)%果實性狀
서과%분자표기%유전도보%수량성상위점(QTL)%과실성상
watermelon (Citrullus lanatus)%molecular marker%genetic map%
quantitative trait loci (QTL)%fruit characteristics
用可溶性固形物含量高、薄皮、感枯萎病的栽培西瓜自交系(Citrulluslanatus var. lanatus)97103和可溶性固形物含量低、皮厚、抗病的野生西瓜种质(Citrullus lanatus var. citroides)PI296341杂交所得F2的118个单株为作图群体,通过构建分子连锁图谱,对西瓜主要果实性状可溶性固形物含量、果皮硬度、果皮厚度、单果重、种子千粒重进行区间作图,定位了影响可溶性固形物含量的4个QTL、影响果皮硬度的5个QTL、影响果皮厚度的2个QTL、影响单果重的3个QTL、影响种子千粒重的6个QTL。此外,估算了每个QTL的贡献率、加性效应与显性效应。为开展西瓜果实性状改良分子标记辅助选择提供了理论基础。
用可溶性固形物含量高、薄皮、感枯萎病的栽培西瓜自交繫(Citrulluslanatus var. lanatus)97103和可溶性固形物含量低、皮厚、抗病的野生西瓜種質(Citrullus lanatus var. citroides)PI296341雜交所得F2的118箇單株為作圖群體,通過構建分子連鎖圖譜,對西瓜主要果實性狀可溶性固形物含量、果皮硬度、果皮厚度、單果重、種子韆粒重進行區間作圖,定位瞭影響可溶性固形物含量的4箇QTL、影響果皮硬度的5箇QTL、影響果皮厚度的2箇QTL、影響單果重的3箇QTL、影響種子韆粒重的6箇QTL。此外,估算瞭每箇QTL的貢獻率、加性效應與顯性效應。為開展西瓜果實性狀改良分子標記輔助選擇提供瞭理論基礎。
용가용성고형물함량고、박피、감고위병적재배서과자교계(Citrulluslanatus var. lanatus)97103화가용성고형물함량저、피후、항병적야생서과충질(Citrullus lanatus var. citroides)PI296341잡교소득F2적118개단주위작도군체,통과구건분자련쇄도보,대서과주요과실성상가용성고형물함량、과피경도、과피후도、단과중、충자천립중진행구간작도,정위료영향가용성고형물함량적4개QTL、영향과피경도적5개QTL、영향과피후도적2개QTL、영향단과중적3개QTL、영향충자천립중적6개QTL。차외,고산료매개QTL적공헌솔、가성효응여현성효응。위개전서과과실성상개량분자표기보조선택제공료이론기출。
In this study, we mapped and characterized quantitative trait loci (QTL) affecting watermelon fruit traits. A total of 118 F2 progenies derived from the cross 97103 (which is a cultivar with higher total soluble solids concentration, thin rind, susceptible to Fusarium wilt disease)×PI296341(which is a
wild germplasm with lower total soluble solids concentration, thick rind, resistant to Fusarium wilt disease) were used to construct a 96-markers map in watermelon. By using interval mapping 4 QTLs for total soluble solids concentration, 5 QTLs for hardness of rind, 2 QTLs for thickness of rind, 3 QTLs for weight of simple fruit, and 6 QTLs for weight of one thousand grain seeds were identified. In addition, the explained variations, additive effects and dominance effects for all detected QTLs were analyzed.