CAJ | 학술논문

目的探讨环孢素A体外抗AF18标记的曼氏血吸虫童虫的作用以及虫体表膜流动性.方法制备童虫,环孢素A体外作用,AF18标记,荧光显微镜观察.结果童虫损伤或死亡,虫体逐渐从绿色变成黄色,虫体表面损伤.结论环孢素A增加童虫AF18的含量,减低童虫表膜流动性.
목적탐토배포소A체외항AF18표기적만씨혈흡충동충적작용이급충체표막류동성.방법제비동충,배포소A체외작용,AF18표기,형광현미경관찰.결과동충손상혹사망,충체축점종록색변성황색,충체표면손상.결론배포소A증가동충AF18적함량,감저동충표막류동성.
Objective To explore the in vitro effect of cyclosporine A on the tegument of juvenile Schistosoma mansoni labeled by AF18 and investigate the effect of cyclosporine A on schistosomula surface membrane fluidity. Methods Preparation of transformed schistosomula, adding cyclosporine A into tubes containing schistosomula and labeling of transformed schistosomula with AF18, then observe schistosomula under fluorescence microscope. Results Schistosomula of different groups labeled by AF 18 were damaged by cyclosporine A in vitro. Conclusion Cyclosporine A increases the uptake of AF18 by schistsomula in vitro which is dose-dependent, and decreases the parasite surface membrane fluidity.