CAJ | 학술논문

我们以往的研究表明,直接在心肌梗塞(myocardialinfarction,MI)动物的心脏缺血区注射胚胎干细胞(embryonic stem cells,ESCs)可以提高其心肌功能,干细胞组织工程学可以使组织再生、修复.本研究旨在观察将ESCs接种到生物降解膜内并移植到梗塞部位的效果.通过结扎小鼠左冠状动脉制作MI模型,将培养3 d的带有小鼠ESCs的聚羟基乙酸膜(polyglycolicacid,PGA)移植到心肌缺血及边缘区表面.实验小鼠分成4组:假手术组、MI组、MI+PGA组、MI+ESC组,移植操作8周后检测血流动力学和心肌功能.MI组的血压和左心室功能显著降低.与MI组和MI+PGA组相比,MI+ESC组的血压和心室功能显著改善,存活率也显著增高,在梗塞区检测到GFP阳性组织,表明ESCs存活,并可能有心肌再生.以上结果表明,移植生物降解膜内的ESCs可修复小鼠梗塞区心肌细胞并提高心脏功能.将ESCs和生物降解材料联合运用可能为修复受损心脏提供一个新的治疗方法.
아문이왕적연구표명,직접재심기경새(myocardialinfarction,MI)동물적심장결혈구주사배태간세포(embryonic stem cells,ESCs)가이제고기심기공능,간세포조직공정학가이사조직재생、수복.본연구지재관찰장ESCs접충도생물강해막내병이식도경새부위적효과.통과결찰소서좌관상동맥제작MI모형,장배양3 d적대유소서ESCs적취간기을산막(polyglycolicacid,PGA)이식도심기결혈급변연구표면.실험소서분성4조:가수술조、MI조、MI+PGA조、MI+ESC조,이식조작8주후검측혈류동역학화심기공능.MI조적혈압화좌심실공능현저강저.여MI조화MI+PGA조상비,MI+ESC조적혈압화심실공능현저개선,존활솔야현저증고,재경새구검측도GFP양성조직,표명ESCs존활,병가능유심기재생.이상결과표명,이식생물강해막내적ESCs가수복소서경새구심기세포병제고심장공능.장ESCs화생물강해재료연합운용가능위수복수손심장제공일개신적치료방법.
Our previous findings demonstrated that directly injecting embryonic stem cells (ESCs) into ischemic region of the heart improved cardiac function in animals with experimental myocardial infarction (MI). Tissue engineering with stem cells may provide tissue creation and repair. This study was designed to investigate the effectiveness of grafting of ESC-seeded biodegradable patch oninfarcted heart. MI in mice was induced by ligation of the left coronary artery. Mouse ESCs were seeded on polyglycolic-acid (PGA)material patches. Three days after culture, an ESC-seeded patch was transplanted on the surface of ischemic and peri-ischemic myocardium. Eight weeks after MI operation and patch transplantation, hemodynamics and cardiac function were evaluated in four (sham-operated, MI, MI + cell-free patch, and MI + ESC-patch) groups of mice. The blood pressure and left ventricular function were significantly reduced in the MI animals. Compared with MI alone and MI + cell-free patch groups, the animals Received MI + ESC seeded patches significantly improved blood pressure and ventricular function. The survival rate of the MI mice grafted with MI +ESC-seeded patches was markedly higher than that in MI alone or MI + cell-free patch animals. GFP-positive tissue was detected in infarcted area with grafting of ESC-seeded patch, which suggests the survivors of ESCs and possible myocardial regeneration. Our data demonstrate that grafting of ESC-seeded bioabsorbable patch can repair infarcted myocardium and improve cardiac function in MI mice. This novel approach of combining stem cells and biodegradable materials may provide a therapeutic modality for repairing injured heart.