CAJ | 학술논문

目的:探讨电针"内关"改善急性心肌缺血的机制.方法:将96只大鼠随机分为假手术组、心肌缺血模型组、心肌缺血模型加电针组,采用结扎冠状动脉左前降支建立急性心肌缺血实验模型,造模成功后,电针双侧"内关".用生化方法检测血清心肌酶活性,逆转录聚合酶链反应(RT-PCR)方法检测心肌中c-fos基因的表达.结果:心肌缺血模型组血清心肌酶活性和心肌c-fos mRNA表达显著高于假手术组(P＜0.05),电针后降低,差异有显著性意义(P＜0.05).结论:电针"内关"改善急性心肌缺血的机制与下调心肌组织c-fos mRNA的表达有关.
목적:탐토전침"내관"개선급성심기결혈적궤제.방법:장96지대서수궤분위가수술조、심기결혈모형조、심기결혈모형가전침조,채용결찰관상동맥좌전강지건립급성심기결혈실험모형,조모성공후,전침쌍측"내관".용생화방법검측혈청심기매활성,역전록취합매련반응(RT-PCR)방법검측심기중c-fos기인적표체.결과:심기결혈모형조혈청심기매활성화심기c-fos mRNA표체현저고우가수술조(P＜0.05),전침후강저,차이유현저성의의(P＜0.05).결론:전침"내관"개선급성심기결혈적궤제여하조심기조직c-fos mRNA적표체유관.
Objective: To investigate the mechanism of improvement effect of electro-acupuncture at acupoint Neiguan (PC 6) on acute myocardial ischemia (AMI). Methods: Ninety-six rats were randomly divided into sham-operation group, myocardial ischemia model group and myocardial ischemia model plus electro-acupuncture group, the rat model of AMI was established by occlusion of the anterior descending branch of the left coronary artery, then electro-acupuncture therapy was performed at bilateral acupoint Neiguan (PC 6). The myocardial enzyme activities in plasma was detected with biochemical method, and the myocardial c-fos gene expression was detected with reverse transcription polymerase chain reaction(RT-PCR) method. Results: The myocardial enzyme activities in plasma and the myocardial c-fos gene expression in myocardial ischemia model group were obviously higher than those in sham group(P＜ 0.05), after electro-acupuncture treatment, the myocardial enzyme activities in plasma and the myocardial c-fos gene expression were decreased,there was a significant difference (P＜ 0.05). Conclusion: The mechanism of improvement effect of electro-acupuncture at acupoint Neiguan (PC 6) on AMI is related to down-regulation of the myocardial c-fos mRNA expression.