农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
4期
60-63
,共4页
谢永芳%蔡应繁%江怀仲%夏玉先%孙全%李生伟%王伯初
謝永芳%蔡應繁%江懷仲%夏玉先%孫全%李生偉%王伯初
사영방%채응번%강부중%하옥선%손전%리생위%왕백초
Cotton%WD40%Characterization
A pair of PCR primers was designed based on the conserved sequences of WD40 family gene of different varieties. The normolization library was screened by PCR methods, the cDNA insert size of positive clones were analyzed by PCR method. A full-length cDNA of WD40 (GenBank accession number: EU219610) in cotton was obtained from sequencing. This gene is 1 796 bp in length, containing an open reading frame encoding 274 amino acids and a stop codon from 35th to 860th position. The bioinformatics characterization indicates that the protein is encoded by WD40 domain. pI and molecular weight of the protein encoded were predicted to be 4.24 and 29 kDa, respectively. The yielded gene was accondingly named as GDRP1. RT-PCR analysis showed that the expression of GDRP1 varied during the gland formation process. These results will be helpful for our further study on the gland formation of cotton.
