CAJ | 학술논문

[目的]分析水稻白叶枯病菌基因组中的串联简单重复序列(simple sequence repeats,SSRs),为其在白叶枯病菌基因组分析和遗传多样性研究提供标记信息.[方法]利用开放的基因组数据库资源,对已完成测序的3个白叶枯病菌菌株KACC10331、MAFF311018和PXO99A进行系统的比较分析,包括基因组中SSR的结构类型、分布、丰度等;并以实例解读了在应用中可能遇到的问题.[结果]在这3个白叶枯病菌菌株的基因组中,由1～6个核苷酸为基序的SSR序列分别有940、926和1 035个;平均每隔5.26、5.34和5.06 kb的距离就有1个大于15 bp的SSR序列.在1～6个核苷酸为基序的SSR序列中,数量最多的是6碱基重复,其次分别是3、5、4、2碱基重复序列,而单碱基重复数目极少.在这3个菌株的基因组中,种类丰富、变异性高的是4、5、6个核苷酸的重复基序,但等位序列上3种菌株间的基序可能不同.[结论]3种白叶枯病菌菌株的SSR在结构类型和分布规律上均具有一定的相似性.
[목적]분석수도백협고병균기인조중적천련간단중복서렬(simple sequence repeats,SSRs),위기재백협고병균기인조분석화유전다양성연구제공표기신식.[방법]이용개방적기인조수거고자원,대이완성측서적3개백협고병균균주KACC10331、MAFF311018화PXO99A진행계통적비교분석,포괄기인조중SSR적결구류형、분포、봉도등;병이실예해독료재응용중가능우도적문제.[결과]재저3개백협고병균균주적기인조중,유1～6개핵감산위기서적SSR서렬분별유940、926화1 035개;평균매격5.26、5.34화5.06 kb적거리취유1개대우15 bp적SSR서렬.재1～6개핵감산위기서적SSR서렬중,수량최다적시6감기중복,기차분별시3、5、4、2감기중복서렬,이단감기중복수목겁소.재저3개균주적기인조중,충류봉부、변이성고적시4、5、6개핵감산적중복기서,단등위서렬상3충균주간적기서가능불동.[결론]3충백협고병균균주적SSR재결구류형화분포규률상균구유일정적상사성.
[Objective]The research aimed to analyze simple sequence repeats in Xanthomonas oryzae pv.oryzae genome and provide mark information of the research in analysis of simple sequence repeats in Xanthomonas oryzae pv.oryzae genome and genetic diversity.[Method] In this study,we analyzed and compared the type,relative distribution and relative density of SSRs in the three Xoo races sequenced,KACC10331,MAFF311018 and PXO99A respectively.And we made an application of the possible problems by examples.[Result] In the three genomes,the SSRs numbers are 940,926 and 1 035,respectively.The sequence of above 15bp SSRs is 5.26 kb、5.34 kb and 5.06 kb or so distance,respectively.Hexa-nucleotide repeats are the most abundant,but mono-nucleotide repeats are rare.Tetra-,penta- and hexa-nucleotide repeats are more abundant and variant,indicating higher mutation rates in these species.[Conclusion] The SSRs type and distribution are similar among the three species.