CAJ | 학술논문

以马铃薯龙引薯一号为试验材料诱导微型薯,研究不同培养条件及不同培养基对诱导微型薯的影响,并对60Cc-γ射线辐照打破微型薯休眠进行了初步研究.结果表明,17℃暗培养条件更加适合微型薯的诱导;在17℃暗培养条件下培养基MS(NH4NO31650mg/L KNO31900mg/L)+5mg/L6BA+80g/L蔗糖比MS培养基诱导微型薯效果更好;低剂量60Co-γ辐照处理能缩短微型薯的休眠期和休眠幅度,且不影响微型薯的芽势,不受样品量的限制,能同时处理大批量样品,可以作为打破微型薯休眠的一种行之有效的办法.
이마령서룡인서일호위시험재료유도미형서,연구불동배양조건급불동배양기대유도미형서적영향,병대60Cc-γ사선복조타파미형서휴면진행료초보연구.결과표명,17℃암배양조건경가괄합미형서적유도;재17℃암배양조건하배양기MS(NH4NO31650mg/L KNO31900mg/L)+5mg/L6BA+80g/L자당비MS배양기유도미형서효과경호;저제량60Co-γ복조처리능축단미형서적휴면기화휴면폭도,차불영향미형서적아세,불수양품량적한제,능동시처리대비량양품,가이작위타파미형서휴면적일충행지유효적판법.
Using potato Longyin Ⅰ as the experiment material to induce the minituber, studied the effects of different culture conditions and different media on inducing Mini-tuber and carried out a preliminary study on breaking dormancy of minituber irradiated by 60Co-γ ray. The results showed that, 17℃ and dark culture conditions more suitable for induction minituber; under 17℃ and dark culture condition medium MS (NH4NO3 1650mg/L KNO3 1900mg / L) +5mg/L6 BA +80g/L sucrose is much better than MS medium for inducing minituber; low doses of 60Co-γ ray irradiation treatment can shorten minituberdormancy period and amplitude, without affecting the bud vitality of minituber, free from restrictions on the sample volume, also could handle large quantities of samples simultaneously, can be used as an ef-fective approach to break dormancy of minituber.