CAJ | 학술논문

为深入了解水稻逆境反应的分子机理和发现新的耐逆相关功能基因,采用Affymetrix水稻表达芯片分析超级稻两优培九母本培矮64S(Oryza sativa L.)不同生长发育时期、不同组织器官全基因组在低温、干旱、高温逆境胁迫下的表达水平,筛选出多个多因子诱导高表达特异基因(待另文发表).OsMsr4是其中一个在多种逆境条件,各生长发育时期与组织器官,其表达量均显著上调的基因,用实时定量PCR方法对其表达水平进行了进一步的分析,所得结果与基因芯片结果基本吻合.用 PCR方法扩增获得长为550 bp全长基因序列,其编码的144个氨基酸残基形成Cys2His2型双锌指结构蛋白,并且锌指结构的α-螺旋区含有植物锌指蛋白特定的保守序列QALGGH.因此,OsMsr4有可能是TFⅢA型锌指蛋白,作为转录因子参与各种环境胁迫应答反应,调控多个逆境相关基因表达.
위심입료해수도역경반응적분자궤리화발현신적내역상관공능기인,채용Affymetrix수도표체심편분석초급도량우배구모본배왜64S(Oryza sativa L.)불동생장발육시기、불동조직기관전기인조재저온、간한、고온역경협박하적표체수평,사선출다개다인자유도고표체특이기인(대령문발표).OsMsr4시기중일개재다충역경조건,각생장발육시기여조직기관,기표체량균현저상조적기인,용실시정량PCR방법대기표체수평진행료진일보적분석,소득결과여기인심편결과기본문합.용 PCR방법확증획득장위550 bp전장기인서렬,기편마적144개안기산잔기형성Cys2His2형쌍자지결구단백,병차자지결구적α-라선구함유식물자지단백특정적보수서렬QALGGH.인차,OsMsr4유가능시TFⅢA형자지단백,작위전록인자삼여각충배경협박응답반응,조공다개역경상관기인표체.
In order to understand the molecular mechanisms of stress responses, and the functions of genes involved in stress tolerance in rice(Oryza sativa L.),gene microarray technology was used to investigate the global genome expression profiles of a male-sterile cultivar, Pei′ai 64S, subjected to cold, drought or heat stresses.Gene expression profiles were obtained from leaf and panicle organs at seedling, booting and heading stages.OsMsr4 was one of the gene highly induced by all stresses in all the development stages and plant organs analyzed. Quantitative real-time PCR analysis confirmed the microarray-derived expression pattern. The gene was cloned and encoded a peptide of 144 amino acids residues. The predicted secondary structure of the peptide includes two Cys2His2 domains, which contain the plant-specific zinc finger motif with a highly conserved sequence: QALGGH. The spacer between the two zinc-finger domains was 32 amino acids. This type of structure was characteristic of DNA-binding zinc finger proteins. Thus, OsMsr4 could probably be a TFⅢA (Transcription Factor ⅢA)type zinc finger protein.