CAJ | 학술논문

[目的]降低生产环草石斛试管苗的成本,为大规模生产种苗提供理论技术依据.[方法]比较白砂糖和分析纯蔗糖、自来水和蒸馏水以及有机物、微量元素、NAA等各成分对环草石斛试管苗生长的影响.[结果]自来水和白砂糖代替蒸馏水和分析纯蔗糖对环草石斛试管苗的生长无显著影响,而更有利于苗的生长;去除培养基中的有机物和微量元素对试管苗生长无明显影响;铁盐和NAA是试管苗生长不可缺少的成分.[结论]在环草石斛的试管苗生产中,自来水可以代替蒸馏水,食用白砂糖可以代替分析纯蔗糖,同时也可以去除培养基中的有机物和微量元素等.
[목적]강저생산배초석곡시관묘적성본,위대규모생산충묘제공이론기술의거.[방법]비교백사당화분석순자당、자래수화증류수이급유궤물、미량원소、NAA등각성분대배초석곡시관묘생장적영향.[결과]자래수화백사당대체증류수화분석순자당대배초석곡시관묘적생장무현저영향,이경유리우묘적생장;거제배양기중적유궤물화미량원소대시관묘생장무명현영향;철염화NAA시시관묘생장불가결소적성분.[결론]재배초석곡적시관묘생산중,자래수가이대체증류수,식용백사당가이대체분석순자당,동시야가이거제배양기중적유궤물화미량원소등.
[Objective] The research aimed to reduce the production cost of Dendrobium loddigesii Rolfe tube-seedlings and provide the theoretical technology basis for the large-scale production of seedlings. [Method] The effects of white granulated sugar, analytical sucrose, tap water, distilled water, organic matter, trace elements and NAA on the growth of D. loddigesii tube-seedlings were compared. [Result]Tap water and white granulated sugar that substituted for distilled water and sucrose resp. had no significant effects on the growth of D. loddigesii tube-seedlings and were more favorable for the growth of seedlings. The removal of organic matter and trace elements had no obvious effect on the growth of seedlings. Malysite and NAA were indispensable components for the seedling growth. [Conclusion] In the production of D. loddigesii tube-seedlings, tap water could substitute for distilled water and white granulated sugar could substitute for pure sucrose. And organic matter and elements and other substances could be removed from the culture medium.