CAJ | 학술논문

背景:采用动态无损伤加载系统对去势大鼠骨组织形态计量学研究的文献报道较少.目的:观察不同强度力学加载对去势大鼠骨组织形态计量学参数的影响.设计、时间及地点:对比观察骨形态计量学指标,于2007-04/08在吉林大学生物医学工程研究室完成.材料:9月龄雌性SD大鼠35只,随机分为假手术组、去势对照组、去势加载1N组、去势加载2N组、去势加载4N组,每组7只.方法:将去势对照组和去势加载组大鼠双侧背部卵巢切除,假手术组背部切口除去少量脂肪后,闭合伤口.大鼠去势1周后,去势加载组采用自行研制的动态无损加载系统对大鼠胫骨两端开始加载,加载载荷分为1,2,4N,15min/d,加载4周.主要观察指标:胫骨近端骨组织形态计量学参数的变化.结果:去势加载组骨小梁面积、骨小梁数量、骨小梁厚度均高于去势对照组(P＜0.05,P＜0.01),去势加载4N组和去势加载2N组大鼠骨小梁面积、骨小梁厚度高于去势对照组(P＜0.001);去势加载4N组骨小梁分离度较去势对照组下降(P＜0.05),随着加载载荷的增加,骨小梁面积、骨小梁数量、骨小梁厚度有增加并接近假手术组的趋势,骨小梁分离度有下降趋势.假手术组单荧光标记表面、双荧光标记表面均低于去势对照组.随着加载载荷的增加,单荧光标记表面、双荧光标记表面、双标间距、骨矿化沉积率均有增加趋势;去势加载2N组和去势加载4N组骨矿化沉积率均明显高于去势对照组(P＜0.05).结论:在1～4N范围内,随着加载载荷的增加,去势大鼠各个骨形态计晕学参数都有优化的趋势,骨组织微观结构有较大的改善,骨量丢失减少,骨质疏松进程减慢.
배경:채용동태무손상가재계통대거세대서골조직형태계량학연구적문헌보도교소.목적:관찰불동강도역학가재대거세대서골조직형태계량학삼수적영향.설계、시간급지점:대비관찰골형태계량학지표,우2007-04/08재길림대학생물의학공정연구실완성.재료:9월령자성SD대서35지,수궤분위가수술조、거세대조조、거세가재1N조、거세가재2N조、거세가재4N조,매조7지.방법:장거세대조조화거세가재조대서쌍측배부란소절제,가수술조배부절구제거소량지방후,폐합상구.대서거세1주후,거세가재조채용자행연제적동태무손가재계통대대서경골량단개시가재,가재재하분위1,2,4N,15min/d,가재4주.주요관찰지표:경골근단골조직형태계량학삼수적변화.결과:거세가재조골소량면적、골소량수량、골소량후도균고우거세대조조(P＜0.05,P＜0.01),거세가재4N조화거세가재2N조대서골소량면적、골소량후도고우거세대조조(P＜0.001);거세가재4N조골소량분리도교거세대조조하강(P＜0.05),수착가재재하적증가,골소량면적、골소량수량、골소량후도유증가병접근가수술조적추세,골소량분리도유하강추세.가수술조단형광표기표면、쌍형광표기표면균저우거세대조조.수착가재재하적증가,단형광표기표면、쌍형광표기표면、쌍표간거、골광화침적솔균유증가추세;거세가재2N조화거세가재4N조골광화침적솔균명현고우거세대조조(P＜0.05).결론:재1～4N범위내,수착가재재하적증가,거세대서각개골형태계훈학삼수도유우화적추세,골조직미관결구유교대적개선,골량주실감소,골질소송진정감만.
BACKGROUND:Few reports are found about the effect of ovariectomized rats' bone histomorphometry parameters using non-destructive dynamic loading system.OBJECTIVE:To observe the effect of different loads situations on the bone histomorphometry parameters in ovariectomized rats.DESIGN,TIME AND SETTING:A controlled observation on the bone histomorphometry was performed in the Biomedical Engineering Laboratory of Jilin University from April 2007 to August 2007.MATERIALS:A total of 35 9-month-old Sprague-Dawley female rats were randomly divided into five groups,including sham operation group,ovariectomized (OVX) control group,OVX loading 1 N group,OVX loading 2 N group,OVX loading 4 N group.There were 7 rats in each group.METHODS:Rats in OVX control group and castration load group were processed into bilateral OVX on the back. The sham operation group only underwent the excision of fat tissues on back,and then sutured. After castration for 1 week,rats were loaded with non-destructive dynamic loading system in the two sides of the tibia,15 minutes a day. The mechanical loads would continue for 4 weeks and the loads were 1N,2N and 4N.MAIN OUTCOME MEASURES:Changes of proximal tibia bone histomorphometry parameters.RESULTS:The area,number and thickness of trabecular bone in OVX loading group were all higher than OVX control group (P＜0.05,P＜0.01).The trabecular bone area and thickness in OVX 4 N and OVX 2 N groups were significantly higher than OVX control group (P ＜ 0.001).There was a downward trend of trabecular separation in OVX 4 N group compared with OVX control group (P ＜ 0.05). With the increasing loads,there was an increasing trend of the area,number and thickness of trabecular bone,which were close to sham-operated group. The trabecular separation was declined. Single fluorescent labeled surface and double fluorescent labeled surfaces in sham operated group were all lower than that in OVX control group. With the increase in loads,the single fluorescent labeled surface,double fluorescent labeled surface,interlabeled width and mineral apposition rate had been shown to increase. The OVX 2 N and OVX 4 N groups exhibited a remarkably higher level of mineral apposition rate than OVX control group (P ＜ 0.05).CONCLUSION:With the increase in load at the range of 1-4 N,all parameters of bone histomorphometry improve in the OVX rats,the bone microstrcture is greatly ameliorated,bone mass loss is reduced and the process of osteoporosis is delayed.