CAJ | 학술논문

目的:探讨CCR7在多器官功能障碍综合征(MODS)脾脏中的表达变化及其对树突状细胞(DC)迁移的影响.方法:用酵母多糖腹腔注射复制小鼠MODS模型,分为正常对照组和实验3～6小时组、24～48小时组、5～7天组及10～12天组.运用免疫组化方法检测CD11c和CD205标记阳性DC在各组小鼠脾脏中分布的变化,用流式细胞术检测CD86/CD11c和CCR7/CD11c标记阳性细胞在脾脏中含量的变化.结果:正常小鼠脾脏DC含量较少,主要分布在脾脏边缘区;在3～6小时组CCR7表达率较正常对照组显著增加,DC含量显著增加、活性增高,并向白髓T细胞区大量迁移;24～48小时组T细胞区中DC含量开始减少,而CCR7表达率升高达到峰值;5～7天组DC与CCR7含量接近正常对照组,边缘区和T细胞区均可见DC分布;10～12天组DC含量再次升高,但多呈不成熟状态,且以边缘区分布为主,CCR7表达率下降.结论:在MODS病程中脾脏DC的含量和分布变化与CCR7的表达率密切相关,CCR7可以作为评估脾脏DC迁移能力及功能活性的重要指标.
목적:탐토CCR7재다기관공능장애종합정(MODS)비장중적표체변화급기대수돌상세포(DC)천이적영향.방법:용효모다당복강주사복제소서MODS모형,분위정상대조조화실험3～6소시조、24～48소시조、5～7천조급10～12천조.운용면역조화방법검측CD11c화CD205표기양성DC재각조소서비장중분포적변화,용류식세포술검측CD86/CD11c화CCR7/CD11c표기양성세포재비장중함량적변화.결과:정상소서비장DC함량교소,주요분포재비장변연구;재3～6소시조CCR7표체솔교정상대조조현저증가,DC함량현저증가、활성증고,병향백수T세포구대량천이;24～48소시조T세포구중DC함량개시감소,이CCR7표체솔승고체도봉치;5～7천조DC여CCR7함량접근정상대조조,변연구화T세포구균가견DC분포;10～12천조DC함량재차승고,단다정불성숙상태,차이변연구분포위주,CCR7표체솔하강.결론:재MODS병정중비장DC적함량화분포변화여CCR7적표체솔밀절상관,CCR7가이작위평고비장DC천이능력급공능활성적중요지표.
Objective:To explore CCR7 expression in splenic dendritic cells and its role in migration and activity of splenic dendritic cells in multiple organ dysfunction syndrome (MODS) in mice.Methods:The MODS model of mice was reproduced by Zymosan injection into peritoneal cavity.The mice were randomly divided into groups of normal,3-6 hours,24-48 hours and 10-12 days post zymosan injection.CD11c and CD205 were analysed by immunohistochemistry;The expression of CD86 and CCR7 of DCs were studied by the flow cytometry analysis.Results:In normal mice,many DC were found at the margin between the red and white pulp.In the 3-6 h and 24-48 h groups,CD86 and CCR7 were strongly up-regulated in the DC,and they distributed in T cells areas.In the 10-12 d group,DC distributed at the margin by the immature form.Conclusion:The CCR7 expression level of DC has close correlations with the migration of DC,CCR7 can be used to evaluate the migration and functional activity of DC in MODS.