CAJ | 학술논문

目的:正常睾丸间质组织中存在一定数量的巨噬细胞群和淋巴细胞,炎症时这些细胞大量增加,这一机制还未完全解明.本研究探讨正常睾丸和系统感染后睾丸中单核细胞趋化蛋白-1(Monocyte chenoattractant protein-1,MCP-1)的表达变化.方法:给予小鼠一次性腹腔注射0.4 μg/kg细菌脂多糖(Lipopolysaccharides,LPS),用半定量RT-PCR和Western blot方法检测3、6、12、24、48小时后单核细胞趋化蛋白-1(MCP-1)mRNA和蛋白质在睾丸中的表达情况,并用组织免疫荧光染色确定MCP-1在睾丸中的部位.结果:MCP-1 mRNA和蛋白质在正常小鼠睾丸中低剂量表达;注射LPS 3小时后MCP-1 mRNA表达水平迅速增加,一直延续到24小时;MCP-1蛋白质表达水平从染毒后12小时开始增加,48小时后仍处于高水平.MCP-1主要在睾丸的间质中表达.结论:正常睾丸中低剂量MCP-1可能起着维持巨噬细胞群在睾丸间质中滞留的作用;系统炎症中,MCP-1在睾丸中的高水平表达可能与吸引单核细胞和巨噬细胞进入间质组织有关.
목적:정상고환간질조직중존재일정수량적거서세포군화림파세포,염증시저사세포대량증가,저일궤제환미완전해명.본연구탐토정상고환화계통감염후고환중단핵세포추화단백-1(Monocyte chenoattractant protein-1,MCP-1)적표체변화.방법:급여소서일차성복강주사0.4 μg/kg세균지다당(Lipopolysaccharides,LPS),용반정량RT-PCR화Western blot방법검측3、6、12、24、48소시후단핵세포추화단백-1(MCP-1)mRNA화단백질재고환중적표체정황,병용조직면역형광염색학정MCP-1재고환중적부위.결과:MCP-1 mRNA화단백질재정상소서고환중저제량표체;주사LPS 3소시후MCP-1 mRNA표체수평신속증가,일직연속도24소시;MCP-1단백질표체수평종염독후12소시개시증가,48소시후잉처우고수평.MCP-1주요재고환적간질중표체.결론:정상고환중저제량MCP-1가능기착유지거서세포군재고환간질중체류적작용;계통염증중,MCP-1재고환중적고수평표체가능여흡인단핵세포화거서세포진입간질조직유관.
Objective:There is a large of population of macrophages resident in the testicular interstitial tissue under normal conditions and they are increased during inflammation.The mechanisms involved are unclear.This study focused on the expression of monocyte chenoattractant protein-1 (MCP-1) in the mouse testis before and after an intraperitoneal injection of LPS.Methods:The expression of MCP-1 in testis was detected by using reverse transcription-polymerase chain reaction and Western blot,the immunofluorescent technique was used to detect the localization of MCP-1 protein in testis.Results:In the normal testis,the expression of MCP-1 mRNA and protein was detectable by RT-PCR and immunofluorescent technique,respectively.The level of testicular MCP-1 mRNA increased dramatically at 3-24 h after LPS treatment,the level of MCP-1 protein increased at 12 h after LPS treatment.The MCP-1 was localized in the testicular interstitial tissue.Conclusion:MCP-1 may play a role in maintaining the resident macrophage population in normal testis and regulating monocyte and macrophage influx in inflammatory testis.