CAJ | 학술논문

目的研究杜仲含药血清诱导骨髓间充质干细胞 (Mesenchymal stem cells,MSCs) 向成骨细胞定向分化过程的蛋白表达差异,分析杜仲诱导MSCs向成骨细胞分化的可能调控机制.方法对杜仲含药血清干预前、干预后第2天、第7天,第14天的细胞提取总蛋白,通过双向电泳分离、PDQuest-7.3.0胶图分析软件进行分析找出差异蛋白,并进行质谱分析、数据库查询建立功能蛋白质组.结果双向电泳分离得到641个蛋白点,选取其中75个点进行质谱分析,经数据库查询得到6个功能蛋白.结论实验认为补肾中药杜仲可能通过上调波形蛋白、核纤层蛋白A的表达促进细胞分化,下调钙网织蛋白表达,释放细胞内钙,参与钙结节的形成,与体内骨矿化过程有关.过氧化物歧化酶、膜联蛋白A6与细胞的活力有关,调节着细胞的分裂增殖与分化,但是否与定向成骨细胞分化有着密切关系尚有待进一步研究.
목적 연구두중함약혈청유도골수간충질간세포 (Mesenchymal stem cells,MSCs) 향성골세포정향분화과정적단백표체차이,분석두중유도MSCs향성골세포분화적가능조공궤제.방법 대두중함약혈청간예전、간예후제2천、제7천,제14천적세포제취총단백,통과쌍향전영분리、PDQuest-7.3.0효도분석연건진행분석조출차이단백,병진행질보분석、수거고사순건립공능단백질조.결과 쌍향전영분리득도641개단백점,선취기중75개점진행질보분석,경수거고사순득도6개공능단백.결론 실험인위보신중약두중가능통과상조파형단백、핵섬층단백A적표체촉진세포분화,하조개망직단백표체,석방세포내개,삼여개결절적형성,여체내골광화과정유관.과양화물기화매、막련단백A6여세포적활력유관,조절착세포적분렬증식여분화,단시부여정향성골세포분화유착밀절관계상유대진일보연구.
Objective To study the protein change in the process of MSCs differentiation to osteoblas guiding by blood serum containing Duzhong(Cortex Eucommiae), and its possible regulation mechanisms.Methods Total protein of cells interfered by blood serum containing Duzhong(Cortex Eucommiae)for 0day, 2days, 7days,14days, was extracted, runouted by two-dimensional electrophoresis and searched for different protein spot by PDQuest-7.3.0 software for analyzing gel picture.Functional proteome was established by mass chromatographic analysis and data base querying. Results 641 protein spots were obtained, chose 75 spots to receive mass chromatographic analysis, and 6 functional protein were obtained by data base querying.Conclusion Chinese drug Duzhong(Cortex Eucommiae)may up-regulate express of vimentin and lamin A to promote cell differentiation, down-regulate express of calreticulin to release intracellular Ca2+ which is related to Ca- nodus and bone mineralization in bodies. SOD and annexin A6 are concerned with corpuscular vigor, regulate corpuscular division growth and differentiation, but whether they have intimate relation with directional differentiation from MSCs to osteoblast is unknown, waiting for further research.