CAJ | 학술논문

目的探索脂肪酸分型方法对布鲁氏菌进行分型的可能性.方法选择90株布鲁氏菌经化学方法提取菌体脂肪酸后,用气相色谱分析,获得布鲁氏菌脂肪酸成分的数据资料.并利用SPSS11.5统计软件对获得的数据资料进行聚类分析.结果布鲁氏菌脂肪酸分型方法将90株布鲁氏菌分为5组:第1组为部分牛种菌;部分羊种菌;部分猪种菌;部分绵羊附睾种;部分变异牛种;部分变异羊种;沙林鼠种标准株.第2组为猪种1、2、3、5型标准株和疫苗株S2;羊种疫苗株M28和Rev.1;绵羊附睾种标准株.第3组为部分羊种;部分变异羊种菌;部分牛种3型和6型;犬种;部分绵羊附睾种.第4组为犬种菌标准株.第5组为部分羊1型;部分变异羊种;部分牛1型;部分猪1型和3型.结论依据布鲁氏菌菌体脂肪酸含量差异可以对布鲁氏菌进行分型;依据19:0CYCLOω8c,18:1ω7c,16:0三种脂肪酸含量差异可以区分猪种布鲁氏菌和犬种布鲁氏菌;脂肪酸分型结果进一步提示犬种布鲁氏菌不只1个生物型;脂肪酸分型结果进一步证实牛3型和牛6型布鲁氏菌高度同源.
목적 탐색지방산분형방법대포로씨균진행분형적가능성.방법 선택90주포로씨균경화학방법제취균체지방산후,용기상색보분석,획득포로씨균지방산성분적수거자료.병이용SPSS11.5통계연건대획득적수거자료진행취류분석.결과 포로씨균지방산분형방법장90주포로씨균분위5조:제1조위부분우충균;부분양충균;부분저충균;부분면양부고충;부분변이우충;부분변이양충;사림서충표준주.제2조위저충1、2、3、5형표준주화역묘주S2;양충역묘주M28화Rev.1;면양부고충표준주.제3조위부분양충;부분변이양충균;부분우충3형화6형;견충;부분면양부고충.제4조위견충균표준주.제5조위부분양1형;부분변이양충;부분우1형;부분저1형화3형.결론 의거포로씨균균체지방산함량차이가이대포로씨균진행분형;의거19:0CYCLOω8c,18:1ω7c,16:0삼충지방산함량차이가이구분저충포로씨균화견충포로씨균;지방산분형결과진일보제시견충포로씨균불지1개생물형;지방산분형결과진일보증실우3형화우6형포로씨균고도동원.
To investigate the the possibility to utilize the cellular fatty acid (CFA) information as a method in Brucella typing, 90 Brucella strains were subjected to the study on CFAs, and all the experimental strains were inoculated on Brucella Agar plates for 48 hours. After that, cells were harvested, saponificated, methylated and extracted to provide fatty acids methylesters for gas chromatography analysis. Based on the CFAs data matrix, dendrogram of 90 experimental strains was generated by SPSS11.5 software package. As shown in the dendrogram, 90 Brucella strains could be divided into 5 clusters. The first cluster included some species of Brucella abortus,Brucella melitensis,Brucella suis, Brucella ovis; and some of the variant strains of Brucella abortus and Brucella melitensis and the typical strain of Brucella neotomae. The second cluster included typical strains of Brucella suis (1,2,3 and 5 types); vaccine strains of Brucella suis S2; vaccine strains of Brucella melitensis M28、Rev.1 and typical strain of Brucella ovis. The third cluster included some of Brucella melitensis; some of the variant strains of Brucella melitensis; some of Brucella abortus(3,6 types); Brucella canis and Brucella ovis. The fourth cluster was the typical strain of Brucella canis.and the fifth cluster included some of Brucella melitensis(1 type); some of Brucella abortus (1 type); some of the variant strains of Brucella melitensis and Brucella suis(1,3 type). It is apparent that CFAs information can be used in brucella typing. and Brucella suis and Brucella canis can be distinguished by the difference in the CFA contents of 3 fatty acids 19:0CYCLOω8c, 18:1ω7c and 16:0. The results of CFAs typing in Brucella species show that Brucella canis includes 2 biovars at least and the high homologization of Brucella abortus (3 type) and Brucella abortus(6 type) can be found.