CAJ | 학술논문

目的观察弓形虫感染妊娠中期的BALB/c小鼠后,对胎盘组织中细胞凋亡相关蛋白Bax、Bcl-2、Fas、FasL及TNF-α表达的影响,探讨弓形虫感染导致细胞凋亡增加的可能机制.方法在妊娠第8 d,实验组经腹腔无菌接种纯化的弓形虫速殖子100个,溶于0.2mL PBS;对照组注射等量无菌PBS(0.01mol/L, pH=7.4).于妊娠第12、14、16、18 d,随机处死实验组和对照组孕鼠各5只,采用免疫组织化学法(immunohistochemistry)检测胎盘组织中细胞凋亡相关蛋白Bax、Bcl-2、Fas、FasL及TNF-α的表达水平.结果免疫组化显示,细胞凋亡相关蛋白在胎盘绒毛和蜕膜组织中均有表达,以胎盘合体滋养细胞表达较多.Bax、Fas、FasL及TNF-α的表达量实验组和对照组均随着妊娠期的延长而增加,相同妊娠天数实验组的表达量均比对照组多.Bcl-2的表达量均随着妊娠期的延长而减少,相同妊娠天数实验组的表达量均比对照组少.结论弓形虫感染妊娠中期的小鼠,可引起胎盘组织中细胞凋亡相关蛋白表达量的改变,进而启动内、外源性的细胞凋亡途径,导致胎盘细胞凋亡增加.
목적 관찰궁형충감염임신중기적BALB/c소서후,대태반조직중세포조망상관단백Bax、Bcl-2、Fas、FasL급TNF-α표체적영향,탐토궁형충감염도치세포조망증가적가능궤제.방법 재임신제8 d,실험조경복강무균접충순화적궁형충속식자100개,용우0.2mL PBS;대조조주사등량무균PBS(0.01mol/L, pH=7.4).우임신제12、14、16、18 d,수궤처사실험조화대조조잉서각5지,채용면역조직화학법(immunohistochemistry)검측태반조직중세포조망상관단백Bax、Bcl-2、Fas、FasL급TNF-α적표체수평.결과 면역조화현시,세포조망상관단백재태반융모화세막조직중균유표체,이태반합체자양세포표체교다.Bax、Fas、FasL급TNF-α적표체량실험조화대조조균수착임신기적연장이증가,상동임신천수실험조적표체량균비대조조다.Bcl-2적표체량균수착임신기적연장이감소,상동임신천수실험조적표체량균비대조조소.결론 궁형충감염임신중기적소서,가인기태반조직중세포조망상관단백표체량적개변,진이계동내、외원성적세포조망도경,도치태반세포조망증가.
To observe the influence of the placental apoptosis on the expression of Bax,Bcl-2, Fas, FasLand TNF-α during the second trimester of pregnancy, mice of experimental group were intraperitoneal injected with 100 purified Toxoplasma gondii tachyzoites added in 0.2mL of PBS, while those of the control group were injected with 0.2 mL of sterile PBS (0.01 mol/L, pH 7.4) in the 8-th day of pregnancy. During the 12, 14, 16 and 18-th days of pregnancy, 5 mice both in experimental and control group were randomly killed and the expression levels of the apoptosis-related proteins Bax, Bcl-2, Fas, FasL and TNF-α in the placental tissues were determined by means of immunohistochemical methods. It was showed that the apoptosis-related protein expressed both in villus and decidua of the placenta, most of which were expressed in syneytiotrophoblast (ST). The positive cells with expression of Bax, Fas, FasL and TNF-α increased along with the increase of the pregnant days in both the experimental group and the control group, and the positive cells with expression of Bcl-2 decreased along with the increase of the pregnant days. It was also demonstrated that the positive cells with expression of Bax, Fas, FasL and TNF-α of the experimental group showed a higher percentage of expression than that of the control group on the same pregnant days, but the positive cells with Bcl-2 expression of the experimental group were fewer than that of the control group. It is concluded that the expression of apoptosis-related protein Bax, Bcl-2, Fas, FasL and TNF-α in the placenta were altered when the pregnant mice were infected with Toxoplasma gondii during the second trimester, which may induce the apoptosis through the endogenic and ectogenic pathway.