CAJ | 학술논문

采用复合蛋白酶(胰蛋白酶、碱性蛋白酶)对大麦虫蛋白进行控制酶解,研究酶解时间、酶活比、底物浓度、酶解温度、pH与加酶量等对酶解反应的影响;采用中心组合试验设计,优化酶解工艺;并对大麦虫酸性多肽清除自由基能力进行了测定评价.结果表明,酶解最佳条件为底物浓度5%,时间120 min,胰蛋白酶∶碱性蛋白酶1∶1,pH 8.71,温度48℃,加酶量16.91 mg/g.该酸性多肽对(O)_2~(-·))、DPPH·自由基有很好的清除作用,具有很强还原力.利用优化工艺制得酸性肽得率为79.05%.
채용복합단백매(이단백매、감성단백매)대대맥충단백진행공제매해,연구매해시간、매활비、저물농도、매해온도、pH여가매량등대매해반응적영향;채용중심조합시험설계,우화매해공예;병대대맥충산성다태청제자유기능력진행료측정평개.결과표명,매해최가조건위저물농도5%,시간120 min,이단백매∶감성단백매1∶1,pH 8.71,온도48℃,가매량16.91 mg/g.해산성다태대(O)_2~(-·))、DPPH·자유기유흔호적청제작용,구유흔강환원력.이용우화공예제득산성태득솔위79.05%.
In order to obtain the bioactive peptides, the barley pest protein was hydrolyzed and the antioxidant of its hydrolyzed liquid were determined. The barley pest protein was hydrolyzed by the mixture of trypsin and alkaline protease, substrate concentration, time, the effects of ratio of protease energy, temperature, pH and dosage of protease on enzymatic reaction were studied, the conditions of enzymatic reaction were optimized by central composite experimental design. Then antioxidative activity of the hydrolyzed liquid were determined by its scavenging activity and reducing activity.The optimum hydrolyzed conditions were: substrate concentration 5%, time 120 min , ratio of protease energy 1∶1, pH 8.71, temperature 48℃and dosage of protease 16.91 mg/g.The hydrolyzed liquid has strong scavenging activity on (O_2~(-·))and DPPH·,and strong reducing activity. Under these conditions, YASP was 79.05%.