CAJ | 학술논문

为明确外源茉莉酸类化合物对落叶松防御物质单宁的诱导作用,利用喷雾处理和香草醛比色测定法,研究不同浓度外源茉莉酸类化合物对2年生兴安落叶松和长白落叶松的诱导作用,及对其针叶内单宁含量变化的影响.喷施茉莉酮(ZJ)、茉莉酸甲酯(MJA)和茉莉酸(JA)后,2种树体针叶内单宁含量增加或减少波动明显,诱导作用可持续10～20天.茉莉酮对兴安落叶松针叶内单宁含量变化的影响较茉莉酸甲酯和茉莉酸显著;但3种茉莉酸类化合物均明显影响长白落叶松针叶内单宁含量的变化.0.01 mmol·L~(-1)茉莉酮、茉莉酸甲酯和1 mmol·L~(-1)茉莉酸诱导兴安落叶松针叶内单宁含量明显变化;0.01 mmol·L~(-1)茉莉酸甲酯和1 mmol·L~(-1)茉莉酸对长白落叶松针叶内单宁含量变化影响显著.3种茉莉酸类化合物诱导兴安落叶松针叶内单宁含量的变化幅度高于长白落叶松.总之,喷施外源茉莉酸类化合物能够诱导落叶松针叶内单宁含量系统增加或减少,并具持效性;2种落叶松对外源茉莉酸类物质的应激反应不同,兴安落叶松的应激反应强度大于长白落叶松.
위명학외원말리산류화합물대락협송방어물질단저적유도작용,이용분무처리화향초철비색측정법,연구불동농도외원말리산류화합물대2년생흥안락협송화장백락협송적유도작용,급대기침협내단저함량변화적영향.분시말리동(ZJ)、말리산갑지(MJA)화말리산(JA)후,2충수체침협내단저함량증가혹감소파동명현,유도작용가지속10～20천.말리동대흥안락협송침협내단저함량변화적영향교말리산갑지화말리산현저;단3충말리산류화합물균명현영향장백락협송침협내단저함량적변화.0.01 mmol·L~(-1)말리동、말리산갑지화1 mmol·L~(-1)말리산유도흥안락협송침협내단저함량명현변화;0.01 mmol·L~(-1)말리산갑지화1 mmol·L~(-1)말리산대장백락협송침협내단저함량변화영향현저.3충말리산류화합물유도흥안락협송침협내단저함량적변화폭도고우장백락협송.총지,분시외원말리산류화합물능구유도락협송침협내단저함량계통증가혹감소,병구지효성;2충락협송대외원말리산류물질적응격반응불동,흥안락협송적응격반응강도대우장백락협송.
In order to teat the induced function of exogenous jasmonates on larch tannins, variation of tannin content in needles of two-year-old Larix gmelinii and L. olgensis seedlings was analyzed by vanillin-colorimetry determination after spraying treatments with the different concentrations of exogenous jasmonates. The results showed after being treated with jasmonic acid (JA ), methyl jasmonate (MJA) and (Z)-jasmone (ZJ), tannin content in needles of the two larch species increased or decreased significantly than the control, and the variation lasted for 10～20 days. ZJ had stronger effect on the variation of tannin content in L. gmelinii needles than MJA and JA, whereas the all three jasmonate significantly affected tannin content in L. olgensis needles. Tannin content in Larix gmelinii needles treated with the 0.01 mmol·L~(-1) ZJ, 0.01 mmol·L~(-1) MJA and 1 mmol·L~(-1) JA changed significantly, while the variation of tannin content in L. olgensis needles was obviously affected by 0.01 mmol·L~(-1) MJA and 1 mmol·L~(-1) JA. Compared with that of tannin content in L. olgensis needles, variational degree of L. gmelinii needles were much higher on inducement of the three jasmonates. It is concluded that the treatments with exogenous jasmonates could induce increase or decrease of tannin content in the larch systemically, and the effects were relatively persistent. The stimulus response of L. gmelinii was stronger than that of L. olgensis to exogenous jasmonates.