中国临床实用医学
中國臨床實用醫學
중국림상실용의학
CHINA CLINICAL PRACTICAL MEDICINE
2010年
1期
1-2
,共2页
软骨细胞%生长板%生长激素%Ⅱ型胶原
軟骨細胞%生長闆%生長激素%Ⅱ型膠原
연골세포%생장판%생장격소%Ⅱ형효원
Chondrocyte%Epiphyseal growth plate%Growth hormone%Collagen Ⅱ
目的 探讨生长激素(growth hormone,GH)对离体培养的大鼠胫骨生长板的软骨细胞Ⅱ型胶原(collagen Ⅱ,Col Ⅱ)表达的影响.方法 采用两步酶消化法分离培养5～8只三周龄大鼠生长板的软骨细胞,用RT-PCR和免疫组化的方法分别检测不同浓度GH对软骨细胞Ⅱ型胶原mRNA和蛋白的表达.结果 GH能促进软骨细胞ColⅡ的表达并呈浓度依赖性,GH在10～500 ng/ml之间能促进ColⅡ的表达,与对照组比较差均有统计学意义(P＜0.05),并且以100 ng/ml时表达最强,随着GH浓度的加大,其促ColⅡ表达的效应逐渐减弱,1000 ng/ml与对照组比较无差异(P＞0.05).结论 GH通过促进软骨细胞ColⅡ的表达能维持软骨细胞的表型,GH在使用过程中不会导致骨龄的增加.
目的 探討生長激素(growth hormone,GH)對離體培養的大鼠脛骨生長闆的軟骨細胞Ⅱ型膠原(collagen Ⅱ,Col Ⅱ)錶達的影響.方法 採用兩步酶消化法分離培養5～8隻三週齡大鼠生長闆的軟骨細胞,用RT-PCR和免疫組化的方法分彆檢測不同濃度GH對軟骨細胞Ⅱ型膠原mRNA和蛋白的錶達.結果 GH能促進軟骨細胞ColⅡ的錶達併呈濃度依賴性,GH在10～500 ng/ml之間能促進ColⅡ的錶達,與對照組比較差均有統計學意義(P＜0.05),併且以100 ng/ml時錶達最彊,隨著GH濃度的加大,其促ColⅡ錶達的效應逐漸減弱,1000 ng/ml與對照組比較無差異(P＞0.05).結論 GH通過促進軟骨細胞ColⅡ的錶達能維持軟骨細胞的錶型,GH在使用過程中不會導緻骨齡的增加.
목적 탐토생장격소(growth hormone,GH)대리체배양적대서경골생장판적연골세포Ⅱ형효원(collagen Ⅱ,Col Ⅱ)표체적영향.방법 채용량보매소화법분리배양5～8지삼주령대서생장판적연골세포,용RT-PCR화면역조화적방법분별검측불동농도GH대연골세포Ⅱ형효원mRNA화단백적표체.결과 GH능촉진연골세포ColⅡ적표체병정농도의뢰성,GH재10～500 ng/ml지간능촉진ColⅡ적표체,여대조조비교차균유통계학의의(P＜0.05),병차이100 ng/ml시표체최강,수착GH농도적가대,기촉ColⅡ표체적효응축점감약,1000 ng/ml여대조조비교무차이(P＞0.05).결론 GH통과촉진연골세포ColⅡ적표체능유지연골세포적표형,GH재사용과정중불회도치골령적증가.
Objective To observe the effect of growth hormone on the expression of collagen Ⅱ of cultured growth plate chondrocytes of rats. Methods Primary chondrocytes from tibial growth plate of 5～8 rats by trypsin-collagenase digestion were cultured in monolayer. RT-PCR and immunocytochemistry were performed to investigate the expression of collagen Ⅱ at the mRNA and protein level, respectively with different concentrations of GH. Results GHenhances the expression of collagen Ⅱ of chondrocytes in vitro in a concentration-dependent fashion. The expression of collagen Ⅱ was obviously between the GH concentration of 10 ng/ml and 500 ng/ml(P＜0.05) , maximally at the concentration of 100 ng/ml, and decreased again after higher concentration of GH. There was no significant difference between 1000 ng/ml group and control group(P＞0.05 ). Conclusion GH can maintain the phenotype of chondrocytes by enhancing the expression of collagen Ⅱ and it will not increase the bone age during clinical practice.
