中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2012年
1期
30-33
,共4页
肝癌%抑癌基因%RUNX3基因%信使核糖核酸
肝癌%抑癌基因%RUNX3基因%信使覈糖覈痠
간암%억암기인%RUNX3기인%신사핵당핵산
Liver cancer%Tumor suppressor gene%RUNX3 gene%mRNA
目的 探讨RUNX3基因在肝癌组织和癌旁组织的mRNA表达水平及其与临床病理因素的相关性.方法 采用RT-PCR检测RUNX3基因在51例肝癌组织及其癌旁组织的mRNA表达水平,分析RUNX3基因mRNA表达与临床病理因素的相关性.结果 RUNX3基因在51例肝癌组织的mRNA表达量相对值为0.4509±0.0963；在相应癌旁组织的mRNA表达量相对值为0.9147±0.0222；两者差异有显著统计学意义(t=33.6087,P＜0.001).RUNX3基因mRNA表达水平在分化程度、浸润周围组织、癌栓、肝内转移等病理因素方面的差异有统计学意义(P＜0.05).在性别、肿瘤直径、肿瘤部位、癌肿出血坏死、组织分型方面的差异无统计学意义(P＞0.05).结论 RUNX3基因在肝癌组织的mRNA表达水平显著低于在癌旁组织.RUNX3基因mRNA低表达与分化程度、浸润周围组织、癌栓、肝内转移等病理因素相关.RUNX3基因可能是肝癌的一种抑癌基因.
目的 探討RUNX3基因在肝癌組織和癌徬組織的mRNA錶達水平及其與臨床病理因素的相關性.方法 採用RT-PCR檢測RUNX3基因在51例肝癌組織及其癌徬組織的mRNA錶達水平,分析RUNX3基因mRNA錶達與臨床病理因素的相關性.結果 RUNX3基因在51例肝癌組織的mRNA錶達量相對值為0.4509±0.0963；在相應癌徬組織的mRNA錶達量相對值為0.9147±0.0222；兩者差異有顯著統計學意義(t=33.6087,P＜0.001).RUNX3基因mRNA錶達水平在分化程度、浸潤週圍組織、癌栓、肝內轉移等病理因素方麵的差異有統計學意義(P＜0.05).在性彆、腫瘤直徑、腫瘤部位、癌腫齣血壞死、組織分型方麵的差異無統計學意義(P＞0.05).結論 RUNX3基因在肝癌組織的mRNA錶達水平顯著低于在癌徬組織.RUNX3基因mRNA低錶達與分化程度、浸潤週圍組織、癌栓、肝內轉移等病理因素相關.RUNX3基因可能是肝癌的一種抑癌基因.
목적 탐토RUNX3기인재간암조직화암방조직적mRNA표체수평급기여림상병리인소적상관성.방법 채용RT-PCR검측RUNX3기인재51례간암조직급기암방조직적mRNA표체수평,분석RUNX3기인mRNA표체여림상병리인소적상관성.결과 RUNX3기인재51례간암조직적mRNA표체량상대치위0.4509±0.0963；재상응암방조직적mRNA표체량상대치위0.9147±0.0222；량자차이유현저통계학의의(t=33.6087,P＜0.001).RUNX3기인mRNA표체수평재분화정도、침윤주위조직、암전、간내전이등병리인소방면적차이유통계학의의(P＜0.05).재성별、종류직경、종류부위、암종출혈배사、조직분형방면적차이무통계학의의(P＞0.05).결론 RUNX3기인재간암조직적mRNA표체수평현저저우재암방조직.RUNX3기인mRNA저표체여분화정도、침윤주위조직、암전、간내전이등병리인소상관.RUNX3기인가능시간암적일충억암기인.
Objective To study the expression of RUNX3 mRNA in primary liver cancer (PHC) tissue and its surrounding normal tissue,and its clinical significance.Methods Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the expression of RUNX3 mRNA in tumor and peritumor tissues in 51 patients with PHC.The relationship between RUNX3 mRNA expression and some clinical pathological parameters was analyzed.Results The relative expression values of RUNX3 mRNA in the tumor tissue and the surrounding normal tissue were 0.4509±0.0963 and 0.9147± 0.0222,respectively.The difference was significant (t=33.6087,P＜0.001).The RUNX3 mRNA expression in tumor tissue correlated with some clinical pathological parameters including low tumor differentiation,positive cancer embolus and intrahepatic invasion and metastasis.The RUNX3 mRNA expression was not correlated with other clinicopathological parameters including gender,cancer diameter,cancer location,hemorrhage and necrosis of cancer,and histotype.Conclusion RUNX3 may be a new tumor suppressor gene for PHC.
