CAJ | 학술논문

目的观察血管束、感觉神经柬植入组织工程骨修复兔股骨大段骨缺损的成骨特点,探讨其对骨修复的影响. 方法 36只新西兰大白兔均制备左侧股骨干1.5 cm节段性骨缺损模型,随机分为三组(n=12),组织工程骨组(A组):自体骨髓基质干细胞复合β-磷酸三钙构建组织工程骨植入骨缺损;血管束植入组(B组):组织工程骨与血管束同时植入骨缺损;感觉神经束植入组(C组):组织工程骨与感觉神经束同时植入骨缺损.各组动物术后1、3、6个月行X线检查及影像学评分,同时每组各处死4只动物行大体及组织学观察.结果影像学评分显示各时间点B组与C组的成骨优于A组,差异有统计学意义(P<0.05);B、C组间成骨差异无统计学意义(P>0.05).组织学观察显示新生骨多出现在血管周围,成骨方式以软骨内成骨为主. 结论血管束、感觉神经束植入组织工程骨的方法能更好地促进组织工程骨成骨及大段骨缺损修复.
목적 관찰혈관속、감각신경간식입조직공정골수복토고골대단골결손적성골특점,탐토기대골수복적영향. 방법 36지신서란대백토균제비좌측고골간1.5 cm절단성골결손모형,수궤분위삼조(n=12),조직공정골조(A조):자체골수기질간세포복합β-린산삼개구건조직공정골식입골결손;혈관속식입조(B조):조직공정골여혈관속동시식입골결손;감각신경속식입조(C조):조직공정골여감각신경속동시식입골결손.각조동물술후1、3、6개월행X선검사급영상학평분,동시매조각처사4지동물행대체급조직학관찰.결과 영상학평분현시각시간점B조여C조적성골우우A조,차이유통계학의의(P<0.05);B、C조간성골차이무통계학의의(P>0.05).조직학관찰현시신생골다출현재혈관주위,성골방식이연골내성골위주. 결론혈관속、감각신경속식입조직공정골적방법능경호지촉진조직공정골성골급대단골결손수복.
Objective To investigate the osteogenesis of the tissue-engineered bone implanted with grafts of vascular and sensory nerve bundles. Methods Models of left femoral defects of 1.5 cm were fabricated in 36 New Zealand white rabbits which were randomized into 3 groups. In Group A, the tissue-engineered bone which had been constructed with autologous bone marrow mesenchymal stem cells plus β-tricalcium phosphate (β-TCP) was implanted into the defects. In group B, the tissue-engineered bone plus graft of vascular bundle was implanted into the femoral defects. In group C, the tissue-engineered bone plus graft of sensory nerve bundle was implanted into the bony defects. Bone formation throughout the defects was assessed radiographically 1, 3 and 6 months after implantation, and at the same time 4 rabbits from each group were sacrificed to evaluate bone formation by gross and histological observations. Results The bone formation in groups B and C was better than in group A (P<0.05), but there was no significant difference between groups B and C (P>0.05). New bone formation occurred mostly around the vessels and chiefly in the form of enchondral ossification. Conclusion Implantation of vascular and sensory nerve bundles into tissue-engineered bone may enhance the esteogenesis of tissue-engineered bone in reconstruction of large bony defects.