白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2010年
7期
388-390
,共3页
许琳琳%郑国光%马翠花%贾海蓉%种靖慧%刘淑艳%林永敏
許琳琳%鄭國光%馬翠花%賈海蓉%種靖慧%劉淑豔%林永敏
허림림%정국광%마취화%가해용%충정혜%류숙염%림영민
膜结合型巨噬细胞集落刺激因子%巨噬细胞%免疫细胞功能性极化%细胞因子%吞噬
膜結閤型巨噬細胞集落刺激因子%巨噬細胞%免疫細胞功能性極化%細胞因子%吞噬
막결합형거서세포집락자격인자%거서세포%면역세포공능성겁화%세포인자%탄서
mM-CSF%Macrophage%Immune functional polarization%Cytokine%Phagocytosis
目的 探讨高表达膜结合型巨噬细胞集落刺激因子(mM-CSF)的血液肿瘤细胞对巨噬细胞的异常活化作用.方法 采用稳定表达mM-CSF的淋巴瘤细胞系Namalwa-M和巨噬细胞系RAW264.7体外共培养体系,以转入空载体的Namalwa-V细胞系共培养体系为对照组.流式细胞术检测RAW264.7的CD206(选择性活化巨噬细胞高表达的表面分子)和细胞内白细胞介素(IL)-6、IL-10、IL-12及TNF-α的表达水平;墨汁吞噬实验检测共培养RAW264.7细胞的功能变化.结果 与Namalwa-M共培养的RAW264.7的CD206表达水平明显升高平均均荧光强度为55.12±3.77,提示其巨噬细胞活性增强;IL.10、TNF-α表达上调(201±6)%、(136±6)%;IL-12、IL-6表达下调(990±528)%、(60 ±26)%;吞噬能力明显提高.结论 高表达mM-CSF的血液肿瘤细胞可异常活化巨噬细胞成为高表达IL-10、TNF-α,低表达IL-12,IL-6的异常活化巨噬细胞.
目的 探討高錶達膜結閤型巨噬細胞集落刺激因子(mM-CSF)的血液腫瘤細胞對巨噬細胞的異常活化作用.方法 採用穩定錶達mM-CSF的淋巴瘤細胞繫Namalwa-M和巨噬細胞繫RAW264.7體外共培養體繫,以轉入空載體的Namalwa-V細胞繫共培養體繫為對照組.流式細胞術檢測RAW264.7的CD206(選擇性活化巨噬細胞高錶達的錶麵分子)和細胞內白細胞介素(IL)-6、IL-10、IL-12及TNF-α的錶達水平;墨汁吞噬實驗檢測共培養RAW264.7細胞的功能變化.結果 與Namalwa-M共培養的RAW264.7的CD206錶達水平明顯升高平均均熒光彊度為55.12±3.77,提示其巨噬細胞活性增彊;IL.10、TNF-α錶達上調(201±6)%、(136±6)%;IL-12、IL-6錶達下調(990±528)%、(60 ±26)%;吞噬能力明顯提高.結論 高錶達mM-CSF的血液腫瘤細胞可異常活化巨噬細胞成為高錶達IL-10、TNF-α,低錶達IL-12,IL-6的異常活化巨噬細胞.
목적 탐토고표체막결합형거서세포집락자격인자(mM-CSF)적혈액종류세포대거서세포적이상활화작용.방법 채용은정표체mM-CSF적림파류세포계Namalwa-M화거서세포계RAW264.7체외공배양체계,이전입공재체적Namalwa-V세포계공배양체계위대조조.류식세포술검측RAW264.7적CD206(선택성활화거서세포고표체적표면분자)화세포내백세포개소(IL)-6、IL-10、IL-12급TNF-α적표체수평;묵즙탄서실험검측공배양RAW264.7세포적공능변화.결과 여Namalwa-M공배양적RAW264.7적CD206표체수평명현승고평균균형광강도위55.12±3.77,제시기거서세포활성증강;IL.10、TNF-α표체상조(201±6)%、(136±6)%;IL-12、IL-6표체하조(990±528)%、(60 ±26)%;탄서능력명현제고.결론 고표체mM-CSF적혈액종류세포가이상활화거서세포성위고표체IL-10、TNF-α,저표체IL-12,IL-6적이상활화거서세포.
Objective To investigate the anti-inflammatory/immune modulatory effects of high-expressing membrane bound M-CSF-hematopoietic malignant cells on macrophages. Methods After coculturing RAW264.7 and murine macrophage cell line with Namalwa-M, a cell line stably expressing mM-CSF, and companing with Nainalwa-V, a cell line stably transfected with the empty vector as the control, flow cytometry was used to detect the expression of the marker of alternatively activated macrophages, CD206, and intracellular expression of IL-10, IL-12, IL-6 and TNF-α to study the immunophenotype of RAW264.7; phagocytic assays to investigate their functional activity in vitro. Results RAW264.7 cocultured with Namalwa-M consistently showed high-level expression of CD206, which indicated activities of these macrophage cells were increased. Furthermore, these RAW264.7 expressing high levels of IL-10, TNF-a and low levels of IL-12, IL-6, as determined by intracellular staining were suggested that the phagocytic activity was increased. Functionally, RAW264.7 cocultured with Namalwa-M showed a higher level of phagocytic activity. Conclusion Macrophage generated in vitro after cocultured with mM-CSF-expressing hematopoietic malignant cell line could be transformed into abnormal macrophage with an immunophenotype defined as IL-10-high, IL-12-low, IL-6-low and TNF-α-high.