CAJ | 학술논문

目的观察胰腺纤维化后结缔组织生长因子(connective tissue growth factor，CTGF)在胰腺组织内的表达，探讨其意义。方法通过高脂饲料诱导大鼠胰腺纤维化模型，16周后处死大鼠，取胰腺组织常规病理检查，天狼猩红染色和免疫组织化学染色检测胰腺纤维化组织胶原蛋白I、α-SMA及CTGF蛋白表达。结果胰腺纤维化后，胰腺小叶和腺泡萎缩，小叶间隙增宽，间质内纤维组织明显增生；胰腺组织内胶原蛋白Ⅰ的合成较正常胰腺明显增加(1207.3±115.5比166.7±78.4，P＜0.01)，α-SMA 表达量较正常胰腺组织增高(1500.2±255.8比57.4±23.2,P＜0.01)，CTGF表达较正常胰腺明显增加(2950.5±431.9比382.2±190.8,P＜0.01)，且胰腺星状细胞(PSCs)大量活化。结论 CTGF是胰腺纤维化的重要作用因子，其作用与PSCs活化密切相关。
목적 관찰이선섬유화후결체조직생장인자(connective tissue growth factor，CTGF)재이선조직내적표체，탐토기의의。방법 통과고지사료유도대서이선섬유화모형，16주후처사대서，취이선조직상규병리검사，천랑성홍염색화면역조직화학염색검측이선섬유화조직효원단백I、α-SMA급CTGF단백표체。결과 이선섬유화후，이선소협화선포위축，소협간극증관，간질내섬유조직명현증생；이선조직내효원단백Ⅰ적합성교정상이선명현증가(1207.3±115.5비166.7±78.4，P＜0.01)，α-SMA 표체량교정상이선조직증고(1500.2±255.8비57.4±23.2,P＜0.01)，CTGF표체교정상이선명현증가(2950.5±431.9비382.2±190.8,P＜0.01)，차이선성상세포(PSCs)대량활화。결론 CTGF시이선섬유화적중요작용인자，기작용여PSCs활화밀절상관。
Objective To observe the expression of connective tissue growth factor (CTGF) in pancreas, and discuss its significance. Methods The pancreatic fibrosis model was induced by high fat diets. The rats were sacrificed 16 weeks later, and the pancreatic tissue was harvested for routine pathologic examinations. Pancreatic collagen fibrosis I was determined by HE and Sirius red staining;α-SMA and CTGF expression were detected by immunohistochemistry. Results After pancreatic fibrosis, pancreatic lobules and acinar atrophy was observed, lobules gap was widened, interstitial fibrous tissue was significantly proliferated, the synthesis of pancreatic collagen fibrosis I was significantly increased when compared with normal pancreas ( 1500.2 + 255.8 vs. 57.4 ± 23.2, P ＜ 0. 01 ), the expression of α-SMA was significantly increased when compared with normal pancreas( 1500.2 + 255.8 vs. 57.4 + 23.2, P ＜ 0. 01 ), and the expression of CTGF was significantly increased when compared with normal pancreas (2950.5 ± 431.9 vs. 382.2 + 190.8, P ＜0.01 ), and there were abundant activated PSCs. Conclusions CTGF participated in the regulation of pancreatic fibrosis development; the function of CTGF was closely related to PSCs activation.