CAJ | 학술논문

目的探讨不同剂量的异丙酚对新生大鼠空间学习记忆功能发育及对内源性神经干细胞增殖的影响.方法 5窝新生SD大鼠按照随机区组化分为对照组(C组)、3种不同剂量异丙酚组(P10组、P50组、P50D组),每组15只.P10、P50组分别单次皮下注射异丙酚10mg/kg、50mg/kg;P50D组先后2次皮下注射异丙酚(50mg/kg);C组注射等容量的脂肪乳剂.3d后用BrdU法检测海马齿状回神经元的增殖;使用水迷宫检测出生后28d的幼鼠空间学习记忆能力.结果免疫组化的检测中,P10组齿状回单位面积含BrdU阳性细胞的数量[(1225±154)个/mm2,P＜0.05 ]明显多于Con组[(840±76)个/mm2],而P50D组[(225±66)个/mm2,P＜0.05]明显减少.水迷宫试验中,P50D组空间探索的潜伏期[(42.68±6.18)s,P＜0.05]明显长于Con组[(15.12±3.43)s],P50D组目标象限停留时间[(32.18±5.38)s,P＜0.05]明显少于Con组[(55.66±8.57)s],而P50组、P10组与Con组的差异均无统计学意义.结论大剂量异丙酚可以抑制新生大鼠的空间学习、记忆能力发育,并可抑制内源性神经干细胞增殖;小剂量的异丙酚反而可以促进内源性神经干细胞增殖.
목적 탐토불동제량적이병분대신생대서공간학습기억공능발육급대내원성신경간세포증식적영향.방법 5와신생SD대서안조수궤구조화분위대조조(C조)、3충불동제량이병분조(P10조、P50조、P50D조),매조15지.P10、P50조분별단차피하주사이병분10mg/kg、50mg/kg;P50D조선후2차피하주사이병분(50mg/kg);C조주사등용량적지방유제.3d후용BrdU법검측해마치상회신경원적증식;사용수미궁검측출생후28d적유서공간학습기억능력.결과 면역조화적검측중,P10조치상회단위면적함BrdU양성세포적수량[(1225±154)개/mm2,P＜0.05 ]명현다우Con조[(840±76)개/mm2],이P50D조[(225±66)개/mm2,P＜0.05]명현감소.수미궁시험중,P50D조공간탐색적잠복기[(42.68±6.18)s,P＜0.05]명현장우Con조[(15.12±3.43)s],P50D조목표상한정류시간[(32.18±5.38)s,P＜0.05]명현소우Con조[(55.66±8.57)s],이P50조、P10조여Con조적차이균무통계학의의.결론 대제량이병분가이억제신생대서적공간학습、기억능력발육,병가억제내원성신경간세포증식;소제량적이병분반이가이촉진내원성신경간세포증식.
Objective To investigate the effects of propofol on the development of spatial learning and memory and neuron proliferation of neonatal rats at different doses. Methods 60 neonatal rats were divided into four groups among per litter by using a randomized block design. Three different doses of propofol group were induced with propofol 10 mg/kg( group P10) ,50 mg/kg( group P50) or 50 mg/kg twice( group P50D) by subcutaneous injection respectively. Neuron proliferation at dentate gyrus was detected by using BrdU marker 3 days later.Morris water maze test was carried out on postnatal day 28. Escape latency,time in probe quadrant were recorded.Results Compared to the control group,neuron marked with BrdU at dentate gyrus in group P50D was significantly decreased( (840±76) vs (225 ±66), P＜0.05) ,group P10 was significantly increased( (840 ±76) vs ( 1225± 154), P＜0.05). Compared to the control group,latency of group P50D was significantly increased( ( 15.12 ±3.43 ) s vs (42.68 ± 6. 18 ) s, P ＜ 0. 05 ), time in probe quadrant of group P50D were significantly decreased ( ( 55.66 ± 8.57 ) s vs (32. 18 ± 5. 38 ) s, P＜ 0. 05 ). Compared to the control group, there was no significant difference between group P50 and group P10. Conclusion Propofol given to seven-day-old rats with 50 mg/kg twice by subcutaneous injection suppresses neuron proliferation and impairs development of memory and learning in neonatal rats,but propofol given with 10 mg/kg once promotes neuron proliferation.