CAJ | 학술논문

目的探讨重组人骨形成蛋白-2(rhBMP-2)在治疗造血损伤中的作用机制,构建探针并通过原位杂交技术观察rhBMP-2治疗小鼠急性造血损伤的作用.方法将22只BALB/c雌性小鼠随机分为照射组和骨形成蛋白治疗组.把正常雄性小鼠的骨髓细胞移植给接受8.5 Gy 60 Co γ射线照射的22只BALB/c雌性小鼠.14 d后,剩余存活的雌性小鼠左股骨再接受9 Gy的电子线照射.骨形成蛋白治疗组小鼠每天每只腹腔注射重组人骨形成蛋白-2 20 mg/kg,连续治疗6 d,照射对照组每天每只腹腔注射等量生理盐水.14 d后,处死小鼠并取股骨制成石蜡切片.用原位杂交技术检测雌性小鼠股骨中雄性供体小鼠的骨髓细胞.结果原位杂交结果显示,照射组小鼠左股骨骨髓中的供体来源细胞数量明显比骨形成蛋白治疗组小鼠左股骨骨髓中多(T=155.0,P＜0.05).而照射组小鼠左股骨骨髓中供体来源细胞数量与2组小鼠右股骨骨髓中相比则无明显差异(T=92.0、78.5,P＞0.05).骨形成蛋白组小鼠左股骨骨髓中的供体来源细胞数量明显比两组小鼠右股骨骨髓中少(T=155.0、55.0,P＜0.05).结论 rhBMP-2促进了自身受损伤的骨髓细胞修复,故在骨形成蛋白组小鼠的左股骨骨髓中几乎检测不到供体雄性小鼠SRY基因的存在.推测rhBMP-2促进了自身骨髓间充质干细胞增殖或分化,并改善造血微环境,加快造血修复、重建的过程.
목적 탐토중조인골형성단백-2(rhBMP-2)재치료조혈손상중적작용궤제,구건탐침병통과원위잡교기술관찰rhBMP-2치료소서급성조혈손상적작용.방법 장22지BALB/c자성소서수궤분위조사조화골형성단백치료조.파정상웅성소서적골수세포이식급접수8.5 Gy 60 Co γ사선조사적22지BALB/c자성소서.14 d후,잉여존활적자성소서좌고골재접수9 Gy적전자선조사.골형성단백치료조소서매천매지복강주사중조인골형성단백-2 20 mg/kg,련속치료6 d,조사대조조매천매지복강주사등량생리염수.14 d후,처사소서병취고골제성석사절편.용원위잡교기술검측자성소서고골중웅성공체소서적골수세포.결과 원위잡교결과현시,조사조소서좌고골골수중적공체래원세포수량명현비골형성단백치료조소서좌고골골수중다(T=155.0,P＜0.05).이조사조소서좌고골골수중공체래원세포수량여2조소서우고골골수중상비칙무명현차이(T=92.0、78.5,P＞0.05).골형성단백조소서좌고골골수중적공체래원세포수량명현비량조소서우고골골수중소(T=155.0、55.0,P＜0.05).결론 rhBMP-2촉진료자신수손상적골수세포수복,고재골형성단백조소서적좌고골골수중궤호검측불도공체웅성소서SRY기인적존재.추측rhBMP-2촉진료자신골수간충질간세포증식혹분화,병개선조혈미배경,가쾌조혈수복、중건적과정.
Objective To investigate the mechanism of recombinant human bone morphogenetic protein-2(rhBMP-2)in repairing hematopoietic injury in mice irradiated with γ-ray.To prepare SRY gene probe and study the effect of rhBMP-2 in repairing hematopoietic injury in mice by in situ hybridization.Methods Twenty-two BALB/c female mice were randomly divided into the irradiated group and BMP treated group,respectively.Bone marrow cells of normal male mice were transplanted into 22 female mice post-irradiation to 8.5 Gy of 60 Co γ rays.The left femurs of the survived female mice were re-irradiated with 9 Gy 14 days later.Mice in BMP treated group were given rhBMP-2 20 mg/kg while those in control group were treated with 0.9% saline by intraperitoneal injection every day for 6 days.These mice were killed 14 days later and paraffin sections of femurs were made.The SRY gene was detected with in situ hybridization.Results There were more positive blots in the left femurs of the mice in irradiated group than those in BMP treated group(T=155.0,P＜0.05).The number of positive blots between the left femurs of the mice in irradiated group and the right femurs of the mice in two groups was not significantly different(T=92.0,78.5,P＞0.05).The number of positive blots in the left femurs of the mice in BMPtreated group was significantly less than those in the right femurs of the mice in two groups(T=155.0,55.0,P＜0.05).Conclusions No donor cell of male mice was detected in the left femurs of BMP treated group,suggesting that rhBMP-2 promoted the restoration of residuary bone marrow cells.Thus,rhBMP-2 promotes the proliferation or differentiation of residuary mesenchymal stem cells,improves hematopoietic microenvironment and accelerates the hematopoietic restoration.