中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2011年
5期
363-366
,共4页
冯劲峰%徐彬%葛海燕%刘新垣
馮勁峰%徐彬%葛海燕%劉新垣
풍경봉%서빈%갈해연%류신원
结肠肿瘤%疾病模型,动物%腺病毒,人%细胞凋亡%Lipocalin 2
結腸腫瘤%疾病模型,動物%腺病毒,人%細胞凋亡%Lipocalin 2
결장종류%질병모형,동물%선병독,인%세포조망%Lipocalin 2
Colonic neoplasms%Disease model,animal%Adenoviruses,human%Apoptosis%Lipocalin 2
目的 探讨携带lipocalin 2基因的溶瘤腺病毒ZD55-lipocalin 2对结肠癌移植瘤的治疗作用.方法 选用SW620细胞构建结肠癌移植瘤模型,随机分为ZD-55组、Ad-lipocalin 2组、ZD55-lipocalin 2组及PBS对照组,瘤内注射相应病毒后观察移植瘤生长及裸鼠生存情况,计算移植瘤瘤重和抑瘤率.通过TUNEL调亡检测、免疫组化检测血管内皮细胞生成因子表达和微血管计数来观察ZD55-lipocalin 2对大肠癌的抑制作用.结果 ZD-55病毒组、Ad-lipocalin 2病毒组和ZD55-lipocalin 2病毒组抑瘤率分别为16.4%、18.9%、40.4%,凋亡指数分别为20.7%、19.3%、77.0%,微血管密度分别为34±6、30±6、11±4.ZD55-lipoealin 2组与其他各组相比差异有统计学意义(P＜0.05).结论 ZD55-lipocalin 2促进大肠癌细胞凋亡、抑制微血管生成,明显抑制大肠癌移植瘤生长.
目的 探討攜帶lipocalin 2基因的溶瘤腺病毒ZD55-lipocalin 2對結腸癌移植瘤的治療作用.方法 選用SW620細胞構建結腸癌移植瘤模型,隨機分為ZD-55組、Ad-lipocalin 2組、ZD55-lipocalin 2組及PBS對照組,瘤內註射相應病毒後觀察移植瘤生長及裸鼠生存情況,計算移植瘤瘤重和抑瘤率.通過TUNEL調亡檢測、免疫組化檢測血管內皮細胞生成因子錶達和微血管計數來觀察ZD55-lipocalin 2對大腸癌的抑製作用.結果 ZD-55病毒組、Ad-lipocalin 2病毒組和ZD55-lipocalin 2病毒組抑瘤率分彆為16.4%、18.9%、40.4%,凋亡指數分彆為20.7%、19.3%、77.0%,微血管密度分彆為34±6、30±6、11±4.ZD55-lipoealin 2組與其他各組相比差異有統計學意義(P＜0.05).結論 ZD55-lipocalin 2促進大腸癌細胞凋亡、抑製微血管生成,明顯抑製大腸癌移植瘤生長.
목적 탐토휴대lipocalin 2기인적용류선병독ZD55-lipocalin 2대결장암이식류적치료작용.방법 선용SW620세포구건결장암이식류모형,수궤분위ZD-55조、Ad-lipocalin 2조、ZD55-lipocalin 2조급PBS대조조,류내주사상응병독후관찰이식류생장급라서생존정황,계산이식류류중화억류솔.통과TUNEL조망검측、면역조화검측혈관내피세포생성인자표체화미혈관계수래관찰ZD55-lipocalin 2대대장암적억제작용.결과 ZD-55병독조、Ad-lipocalin 2병독조화ZD55-lipocalin 2병독조억류솔분별위16.4%、18.9%、40.4%,조망지수분별위20.7%、19.3%、77.0%,미혈관밀도분별위34±6、30±6、11±4.ZD55-lipoealin 2조여기타각조상비차이유통계학의의(P＜0.05).결론 ZD55-lipocalin 2촉진대장암세포조망、억제미혈관생성,명현억제대장암이식류생장.
Objective To investigate the antitumor activity of the oncolytic adenovirus expressing lipocalin 2 gene for colorectal cancer in vivo.Methods BALB/C nude mice subcutaneously inoculated by SW620 cells and grown tumors were treated with injection of ZD-55 virus, Ad-lipocalin 2 virus and ZD55- lipocalin 2 virus respectively.The weight of implanted tumors and the tumor inhibition rate were calculated to evaluate the anti-tumor effect.Cell apoptosis was determined by TUNEL and the protein expression of VEGF and MVD were determined with immunohistochemistry.Results ZD55-lipocalin 2 inhibited the growth of transplanted tumor more significantly than ZD-55 virus and Ad-lipocalin 2 virus ( P ＜ 0.05 ).Tumor cell apoptosis was upregulated and the MVD reduced significantly in ZD55-lipocalin 2 group in contrast to the other two groups (P ＜0.05).Conclusions ZD55-lipocalin 2 induces apoptosis of colorectal tumor cells and inhibits tumor microvascular formation, slowing down the growth of transplantation tumors.
