CAJ | 학술논문

目的探讨2008-2009年广东省手足口病非CAI6非EV71肠道病毒株的流行情况以及毒株型别.方法从2008-2009年广东省手足口病粪便样本中采用RD细胞和HEp-2细胞分离病毒毒株,待出现细胞病变后收集上清采用RT-PCR进行鉴定.非CA16非EV71毒株再进行VP1测序分析,序列通过BLAST程序鉴定型别,用MEGA4.0软件进行基因进化分析.结果共分离到22株非CA16非EV71毒株,通过BLAST程序鉴定犁别.2008年9株非CA16、非EV71的毒株有CA2型、CA4、CB3型；2009年13株有EV80、E13、E30、CB5、E24、PV1、CA10、CA6和CA2.各毒株间同源性低,各毒株分别归属CoxA、CoxB、埃可肠道病毒、新型肠道病毒和脊灰病毒组.结论广东省2008-2009年两年来,手足口病除了CA16和EV71占主导地位外,并不存在其他的优势株,其他型肠道病毒分布比较广,既有CoxA组病毒、CoxB组,也有E13、E24、E30、新型病毒EV80和脊髓灰质炎病毒PV1,儿组病毒伴随流行.
목적 탐토2008-2009년광동성수족구병비CAI6비EV71장도병독주적류행정황이급독주형별.방법 종2008-2009년광동성수족구병분편양본중채용RD세포화HEp-2세포분리병독독주,대출현세포병변후수집상청채용RT-PCR진행감정.비CA16비EV71독주재진행VP1측서분석,서렬통과BLAST정서감정형별,용MEGA4.0연건진행기인진화분석.결과 공분리도22주비CA16비EV71독주,통과BLAST정서감정리별.2008년9주비CA16、비EV71적독주유CA2형、CA4、CB3형；2009년13주유EV80、E13、E30、CB5、E24、PV1、CA10、CA6화CA2.각독주간동원성저,각독주분별귀속CoxA、CoxB、애가장도병독、신형장도병독화척회병독조.결론 광동성2008-2009년량년래,수족구병제료CA16화EV71점주도지위외,병불존재기타적우세주,기타형장도병독분포비교엄,기유CoxA조병독、CoxB조,야유E13、E24、E30、신형병독EV80화척수회질염병독PV1,인조병독반수류행.
Objective To discuss the prevalence of non-EV71,non-CA16 virus strains of hand,foot and mouth disease(HFMD) in Guangdong province between 2008 and 2009,and analyze the genetic evolution of these non-EV71,non-CA16 virus strains.Methods Isolated viruses from stool samples collected from outpatient and in-patient cases of HFMD between 2008 and 2009 by human rhabdomyosarcoma(RD) cell and HEp-2 cell,cultures that exhibited a characteristic enterovirus cytopathic effect were evaluated by RT-PCR.Those strains which identified non-EV71,non-CA16 were analyzed by VP1 sequencing and then were identified by BLAST program.A phylogenetic tree was constructed using the Neighor-Joinning method in the MEGA 4.0 software.Results Twenty-two virus strains of non-EV71,non-CA16 were obtained,and nine of the twenty-two virus strains in 2008 were classified into CA2,CA4,and CB3 by BLAST; thirteen of the twenty-two virus strains in 2009 were classified into EV80,Echo13,Echo30,CBS,Echo24,CA10,CA6,and poliovirus 1 by BLAST.The honology of all strains was low,and all the strains belonged to CA,CB,Echoviruses,Enterovirus and poliovirus subgroup.Conclusion Except for EV71 and CA16 was a major causative agent in prevail of HFMD in Guangdong province between 2008 and 2009,there also existed other subgroup Enterovirus.The other twenty-two strains respectively belonged to CA,CB,Echoviruses,Enterovirus and poliovirus subgroup,and none of those strains was predominant.Muti-species Enterovirus occurred concomitantly.