CAJ | 학술논문

目的分析杀伤细胞免疫球蛋白样受体(KIR)基因型和单倍型在强直性脊柱炎患者中的分布特点.方法采用序列特异性引物聚合酶链反应方法 ,分析105例强直性脊柱炎患者、62例骨关节炎患者和412名健康对照KIR基因型和单倍型.结果 (1)强直性脊柱炎组KIR基因型3DL3-2DL3-2DL1-2DP1-2DL4-3DL1-2DS4-3DL2阳性率(6.67%)明显低于健康对照组和骨关节炎组(20.15%,17.74%;P=0.001,0.037).(2)强直性脊柱炎组KIR基因型3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2阳性率(9.52%)及基因型3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2阳性率(5.71%)明显高于健康对照组(2.18%,0.49%;P=0.001,0.001),骨关节炎组未检出这2种基因型.(3)三组人群KIR单倍型差异无统计学意义.结论 KIR基因型分布异常,可能是强直性脊柱炎的免疫遗传因素,其分子机制有待进一步研究.
목적 분석살상세포면역구단백양수체(KIR)기인형화단배형재강직성척주염환자중적분포특점.방법 채용서렬특이성인물취합매련반응방법 ,분석105례강직성척주염환자、62례골관절염환자화412명건강대조KIR기인형화단배형.결과 (1)강직성척주염조KIR기인형3DL3-2DL3-2DL1-2DP1-2DL4-3DL1-2DS4-3DL2양성솔(6.67%)명현저우건강대조조화골관절염조(20.15%,17.74%;P=0.001,0.037).(2)강직성척주염조KIR기인형3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2양성솔(9.52%)급기인형3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2양성솔(5.71%)명현고우건강대조조(2.18%,0.49%;P=0.001,0.001),골관절염조미검출저2충기인형.(3)삼조인군KIR단배형차이무통계학의의.결론 KIR기인형분포이상,가능시강직성척주염적면역유전인소,기분자궤제유대진일보연구.
Objective To investigate the association of killer cell immunoglobulin-like receptors (KIR) genotype and haplotype with ankylosing spondylitis (AS). Methods Peripheral blood samples were collected from 105 AS patients, 62 patients of osteoarthritis (OA), and 412 randomly selected healthy controls. Polymerase chain reaction with sequence-specific primers (PCR-SSP) was used to detect the KIR genotype and haplotype. Results The genotype frequency of 3DL3-2DL3-2DL1-2DP1-2DLA-3DL1-2DS4-3DL2 (6.67%)was significantly lower in the AS patients than in the control subjects (20.15% ) and OA patients( 17.74%, P = 0. 001,0. 037 respectively). The genotype frequency of 3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS2-2DS3-2DS4-2DS5-3DS1-3DL2 and 3DL3-2DL3-2DL2-2DL1-2DP1-2DL4-3DL1-2DL5-2DS1-2DS4-3DL2 of the AS patients (9.52% ,5.71% )was significantly higher than that of the controls (2.18 % ,0.49 % ; P = 0.001,0.001 ), and these two genotypes were not detected in the OA patients. There were not significant differences in the haplotypes A and B among the AS patients, OA patients, and healthy controls. Conclusion KIR genotypes may be associated with the susceptibility to AS.