CAJ | 학술논문

目的探讨食管鳞癌患者外周血循环肿瘤细胞数量与食管癌病理生理特征之间的关系.方法采取负性筛选策略,红细胞裂解液去除食管鳞癌外周血样本中的红细胞,Miltenyi免疫磁珠去除白细胞,富集上皮来源的稀有细胞,免疫荧光染色技术鉴定循环肿瘤细胞.结果食管鳞癌患者外周血循环肿瘤细胞的数量与患者性别、年龄、肿瘤位置均无显著相关性,与细胞分化程度、原发肿瘤侵犯程度、淋巴结转移状态、P-TNM分期有显著相关,相关系数分别为0.504、0.507、0.412、0.374.结论食管鳞癌患者外周血循环肿瘤细胞的数量可以反映肿瘤的进展程度,可作为判断食管鳞癌生物学行为恶化的指标.
목적 탐토식관린암환자외주혈순배종류세포수량여식관암병리생리특정지간적관계.방법 채취부성사선책략,홍세포렬해액거제식관린암외주혈양본중적홍세포,Miltenyi면역자주거제백세포,부집상피래원적희유세포,면역형광염색기술감정순배종류세포.결과 식관린암환자외주혈순배종류세포적수량여환자성별、년령、종류위치균무현저상관성,여세포분화정도、원발종류침범정도、림파결전이상태、P-TNM분기유현저상관,상관계수분별위0.504、0.507、0.412、0.374.결론 식관린암환자외주혈순배종류세포적수량가이반영종류적진전정도,가작위판단식관린암생물학행위악화적지표.
Objective To explore the relationship between circulating tumor cells(CTCs) in the peripheral blood of patients with esophageal squamous cell carcinoma(ESCC) and the physiopathological characteristics of esophageal neoplasms.Methods Using negative selection system,we depleted red blood cells(RBCs) in red blood cell lysis buffer,depleted white blood cells (WBCs) with Miltenyi magnetic beads and enriched the rare cells from ESCC patients'peripheral blood.Immunofluorence staining (IF) was adopted to identify CTCs.Results Circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma was closely related to cell differentiation grade,the invasion of primary cancer,lymph node status,P-TNM stages,and was rarely related to the sex,age or the location of tumor.Conclusion The results suggest that circulating tumor cells in the peripheral blood of patients with esophageal squamous cell carcinoma may express the development of esophageal cancer and may be served as a tumor marker to evaluate the biological behavior of esophageal cancer.