CAJ | 학술논문

目的研究强脉冲光(intense pulse light,IPL)对长波紫外线(ultraviolet A,UVA Ⅰ)诱导的正常离体人皮肤成纤维细胞(fibroblast,FB)损伤的保护作用,探讨以IPL为手段的光子嫩肤技术的理论基础.方法分离并培养人FB,用不同剂量UVA Ⅰ照射FB以诱导细胞光损伤,CCK-8检测其增殖能力的情况,根据预实验结果确定IPL剂量进行照射,流式细胞仪技术枪测细胞周期,Western印迹法检测CylinD1和CDK2蛋白的表达水平.结果不同剂量的UVA Ⅰ可造成FB的损伤,随着UVA Ⅰ剂量的增加,细胞增殖能力下降,11 J/cm2的剂量可明显导致细胞大量死亡,而7 J/cm2的UVAⅠ对细胞的增殖能力没有明显的影响;经UVA Ⅰ照射2 d后再进行IPL连续照射2 d,细胞增殖活性高于单独UVA Ⅰ处理组,差异具有统计学意义.流式细胞仪检测结果表明该组细胞增殖指数升高.细胞周期蛋白CyclinD1和CDK2的表达水平升高.结论 IPL可通过调节周期蛋白的表达而促进正常FB增殖,从而保护UVAⅠ诱导的FB光损伤,为应用IPL面部美容除皱即光子嫩肤术提供理论依据.
목적 연구강맥충광(intense pulse light,IPL)대장파자외선(ultraviolet A,UVA Ⅰ)유도적정상리체인피부성섬유세포(fibroblast,FB)손상적보호작용,탐토이IPL위수단적광자눈부기술적이론기출.방법 분리병배양인FB,용불동제량UVA Ⅰ조사FB이유도세포광손상,CCK-8검측기증식능력적정황,근거예실험결과학정IPL제량진행조사,류식세포의기술창측세포주기,Western인적법검측CylinD1화CDK2단백적표체수평.결과 불동제량적UVA Ⅰ가조성FB적손상,수착UVA Ⅰ제량적증가,세포증식능력하강,11 J/cm2적제량가명현도치세포대량사망,이7 J/cm2적UVAⅠ대세포적증식능력몰유명현적영향;경UVA Ⅰ조사2 d후재진행IPL련속조사2 d,세포증식활성고우단독UVA Ⅰ처리조,차이구유통계학의의.류식세포의검측결과표명해조세포증식지수승고.세포주기단백CyclinD1화CDK2적표체수평승고.결론 IPL가통과조절주기단백적표체이촉진정상FB증식,종이보호UVAⅠ유도적FB광손상,위응용IPL면부미용제추즉광자눈부술제공이론의거.
Objective To study the protective effects of intense pulse light (IPL) on the injury of normal human skin fibroblasts (FB) induced by ultraviolet A (UVA Ⅰ ) in vitro and to explore its possible mechanism. Methods The human skin fibroblasts were isolated and cultured, and then irradiated by UVA Ⅰ (9 J/cm2) and IPL (15 J/cm2), respectively. The proliferative ability of the cells were detected by CCK-8. Cell cycle was detected by flow cytometry, and cylin D1 and CDK2 protein expression levels were detected by Western blot. Results Different doses of UVA Ⅰ irradiation caused certain damages of cultured fibroblasts. With the increasing of of UVA Ⅰ dose, cell proliferation was decreased. Cells went to death at the exposure to 11 J/cm2 UVA Ⅰ , while the proliferative activity did not change much at 7 J/cm2 UVA Ⅰ . Cells were treated with UVA Ⅰ for other 2 days, then with IPL irradiation for other 2days, showing clear stimulating to the cell proliferation as compared with the cells that received UVA Ⅰ treatment only. Flow cytometry results showed that an increase of cell proliferating index, and cell cycle protein cyclin D1 and CDK2 expression levels were also upregulated after IPL irradiation.Conclusion UVA Ⅰ irradiation may cause cell damage as showed by cell growth index, cyclin D1 and CDK2 expression, and this injury could be protected partly by IPL treatment. The intense pulsed light may regulate the expression of cyclin proteins that may promote normal fibroblast proliferation, which could be one of the mechanisms of IPL skin rejuvenation.