CAJ | 학술논문

目的体外观察碱性成纤维细胞生长因子(basie fibroblast growth factor,bFGF)聚乳酸纳米缓释微球对人脂肪干细胞增殖和成脂分化的影响,为bFGF缓释微球应用于脂肪组织工程的研究提供理论依据.方法体外分离培养脂肪干细胞,并行多向诱导分化鉴定.配制含有0、1、2、3、4、5 mg/ml bFGF聚乳酸缓释微球的脂肪干细胞培养液及成脂分化诱导液.将脂肪干细胞接种至96孔板,第2天更换含不同浓度hFGF缓释微球的培养液和成脂诱导液,分别用四甲基偶氮噻唑蓝比色法(MTT)和油红O定量检测法定期检测细胞增殖和成脂分化的情况.所得数据均用SPSS13.0软件进行统计学处理.结果 bFGF聚乳酸缓释微球有明显促进脂肪干细胞增殖和成脂分化的作用.增殖实验和成脂诱导实验合适的作用浓度分别为3 mg/ml和4 mg/ml.结论 bFGF聚乳酸纳米缓释微球体外可以明显促进脂肪下细胞的增殖和成脂分化,可作为一种较理想的细胞因子缓释系统应用于脂肪组织工程的研究.
목적 체외관찰감성성섬유세포생장인자(basie fibroblast growth factor,bFGF)취유산납미완석미구대인지방간세포증식화성지분화적영향,위bFGF완석미구응용우지방조직공정적연구제공이론의거.방법 체외분리배양지방간세포,병행다향유도분화감정.배제함유0、1、2、3、4、5 mg/ml bFGF취유산완석미구적지방간세포배양액급성지분화유도액.장지방간세포접충지96공판,제2천경환함불동농도hFGF완석미구적배양액화성지유도액,분별용사갑기우담새서람비색법(MTT)화유홍O정량검측법정기검측세포증식화성지분화적정황.소득수거균용SPSS13.0연건진행통계학처리.결과 bFGF취유산완석미구유명현촉진지방간세포증식화성지분화적작용.증식실험화성지유도실험합괄적작용농도분별위3 mg/ml화4 mg/ml.결론 bFGF취유산납미완석미구체외가이명현촉진지방하세포적증식화성지분화,가작위일충교이상적세포인자완석계통응용우지방조직공정적연구.
Objective To investigate the effect of basic fibroblast growth factor-polyactide release nanospheres on proliferation and adipogenic induction of adipose-derived stem cells in vitro.Methods Adipose-derived stem cells were isolated and induced for three-line differentiation in vitro.The culture medium and inductive medium of stem cells were prepared containing 0,1,2,3,4 and 5 mg/ml basic fibroblast growth factor-polyactide release nanospheres,respectively.Adipose-derived stem cells were cultured ina 96-well plate and replaced the culture medium containing release nanospheres the second day.The cells proliferation was detected by the method of MTT every day and quanti fication of oil red O every other day.The data obtained were analyzed with SPSS13.0 statistical software.Results The basic fibroblast growth factor polyactide release nanospheres had the ability promoting proliferation and adipogenic induction of adipose-derived stem cells.The best concentration of nanospheres was 3 mg/ml and 4 mg/ml,respectively.Conclusions The basic fibroblast growth factor-polyactide release nanospheres could promote proliferation and adipogenic induetionof adipose-derived stem cells significantly.It could be used as an ideal cytokine release nanospheres in adipose tissue engineering.