CAJ | 학술논문

背景:生长相关蛋白(GAP-43),勿动蛋白A(Nogo-A)与中枢神经损伤后的再生修复有密切关系,但其基因表达与神经行为功能的关系研究较少.目的:观察大鼠脑缺血再灌注后GAP-43和Nogo-A基因表达与神经行为功能的关系.设计:随机对照的基础实验研究.地点和对象:本实验在青岛大学医学院脑血管病研究所和山东省脑血管病防治重点实验室完成.成年健康雌性SD大鼠36只,体质量230～280 g,清洁级,由中国科学院上海实验动物中心提供.干预:以线栓法建立大脑中动脉缺血再灌注模型.应用Bederson神经功能评分法评定脑缺血再灌注后神经行为功能的恢复,原位杂交技术检测脑组织GAP-43 mRNA和Nogo-A mRNA的表达.主要观察指标:脑缺血再灌注后神经行为功能,脑组织GAP-43 mRNA和Nogo-A mRNA的表达.结果:脑缺血再灌注后神经功能评分于再灌注2～14 d降低,与缺血再灌注2 h比较,差异有显著性意义.在皮质区和纹状体区,脑缺血后GAP-43 mRNA表达(阳性细胞数/视野).于再灌注12 h和2 d出现"双峰"升高现象,以后逐渐降低,再灌注14 d,恢复至假手术组水平.No-go-A mRNA表达(阳性细胞数/视野).也于12 h和2 d出现"双峰"增高,但于再灌注7 d降至假手术组水平.结论:GAP-43 mRNA表达增高和Nogo-A mRNA表达早期升高、晚期下降,可能有助于脑缺血损伤后的神经功能恢复.
배경:생장상관단백(GAP-43),물동단백A(Nogo-A)여중추신경손상후적재생수복유밀절관계,단기기인표체여신경행위공능적관계연구교소.목적:관찰대서뇌결혈재관주후GAP-43화Nogo-A기인표체여신경행위공능적관계.설계:수궤대조적기출실험연구.지점화대상:본실험재청도대학의학원뇌혈관병연구소화산동성뇌혈관병방치중점실험실완성.성년건강자성SD대서36지,체질량230～280 g,청길급,유중국과학원상해실험동물중심제공.간예:이선전법건립대뇌중동맥결혈재관주모형.응용Bederson신경공능평분법평정뇌결혈재관주후신경행위공능적회복,원위잡교기술검측뇌조직GAP-43 mRNA화Nogo-A mRNA적표체.주요관찰지표:뇌결혈재관주후신경행위공능,뇌조직GAP-43 mRNA화Nogo-A mRNA적표체.결과:뇌결혈재관주후신경공능평분우재관주2～14 d강저,여결혈재관주2 h비교,차이유현저성의의.재피질구화문상체구,뇌결혈후GAP-43 mRNA표체(양성세포수/시야).우재관주12 h화2 d출현"쌍봉"승고현상,이후축점강저,재관주14 d,회복지가수술조수평.No-go-A mRNA표체(양성세포수/시야).야우12 h화2 d출현"쌍봉"증고,단우재관주7 d강지가수술조수평.결론:GAP-43 mRNA표체증고화Nogo-A mRNA표체조기승고、만기하강,가능유조우뇌결혈손상후적신경공능회복.
BACKGROUND: Growth associated protein-43 (GAP-43)and Nogo protein-A (Nogo-A) have close relation to the regeneration and reparation following central nervous system injury, however, there are only a few researches on the relationship between gene expression and nervous behavioral function.OBJECTIVE: To observe the relationship between gene expressions of GAP-43 and Nogo-A after cerebral ischemic reperfusion and nervous behavioral function in rats.DESIGN: Randomized controlled basic experiment.SETTING and PARTICIPANTS: This experiment was finished in the Institute of Cerebrovascular Diseases, Medical College of Qingdao University and the Key Laboratory of Cerebrovascular Diseases of Shandong Province.Totally 36 healthy adult female Sprague-Dawley(SD)rats, weighing 230 g to 280 g, clear grade, were purchased from Shanghai Experimental Animal Center of Chinese Academy of Sciences.INTERVENTIONS: The model of focal ischemic reperfusion in SD rats was induced by intraluminal middle cerebral artery occlusion with a nylon monofilament suture. Bederson's neurological grade evaluation was used to examine the nervous behavioral function recovery, in situ hybridization was performed to examine the expression of GAP-43 mRNA and Nogo-A mRNA in the brain tissue.MAIN OUTCOME MEASURES: Nervous behavioral function, the expression of GAP-43 mRNA and Nogo-A mRNA in brain tissue after cerebral ischemic reperfusion.RESULTS: Neurological function score was decreased 2 - 14 days after the cerebral ischemic reperfusion, which had significant difference as compared with that 2 hours' ischemid reperfusion. In the cortex and striatum, GAP-43 mRNA(positive cells number/visual field) expressed as "double-peak" increase 12 hours and 2 days after reperfusion, and then decreased gradually to the level of the sham-operation group 14 days after reperfusion. Nogo-A mRNA expression(positive cells number/visual field) also increased as "double-peak" at 12 hours and 2 days, but reduced to the level of sham-operation group 7 days after reperfusion.CONCLUSION: Neurological function recovery after cerebral ischemic injury may be due to the upregulation of GAP-43 mRNA and the increase in the early period and decrease in the later period of Nogo-A mRNA.