中德临床肿瘤学杂志（英文版）
中德臨床腫瘤學雜誌（英文版）
중덕림상종류학잡지（영문판）
THE CHINESE-GERMAN JOURNAL OF CLINICAL ONCOLOGY
2005年
4期
238-243
,共6页
顾霞%林汉良%邵建勇%张萌%梁惠珍
顧霞%林漢良%邵建勇%張萌%樑惠珍
고하%림한량%소건용%장맹%량혜진
survivin%淋巴瘤%分类%RT-PCR
survivin%淋巴瘤%分類%RT-PCR
survivin%림파류%분류%RT-PCR
survivin%lymphoma%classification%reverse transcription-polymerase chain reaction
目的淋巴瘤的诊断与分型是临床病理诊断的难点.本研究检测抗凋亡基因survivin在不同类型淋巴瘤中的表达,并探讨其对淋巴瘤分型的意义.方法用免疫组化法检测中山大学附属一院及肿瘤医院2001年1月～2003年6月219例淋巴瘤、13例淋巴结反应性增生石蜡标本中survivin基因的表达;同时用RT-PCR检测K562、HL60、Raji、Jurkat细胞系和以上病例中18例淋巴瘤及2例淋巴结反应性增生新鲜标本中survivin mRNA的表达;对不同类型的淋巴瘤survivin蛋白及mRNA表达的结果进行半定量分析.结果 Survivin蛋白在非霍奇金淋巴瘤(NHL)的弥漫性大B细胞性淋巴瘤(DLBL)(88.6％,70/79)、伯基特淋巴瘤(BL)(100％,2/2)、淋巴母细胞淋巴瘤(LBL)(92.3％,12/13)中有较高的表达,而在滤泡性淋巴瘤(FL)(18.2％),粘膜相关性结外边缘带B细胞淋巴瘤(MAL-oma)(40.9％)和MZL(marginal zone lymphoma)(33.3％)中表达较低,且多为弱阳性.高表达组(DLBL、BL、LBL)与低表达组(FL、MZL、MALT)之间差异有统计学意义,χ2检验,P＜0.01.霍奇金淋巴瘤(HL)中大部分R-S(Reed-Sternberg)细胞表达survivin蛋白.NHL中survivin mRNA的检测结果与其蛋白水平的表达呈正相关(相关系数r=0.6270,P＜0.01).结论 survivin蛋白及mRNA表达水平在不同类型淋巴瘤存在着明显的差异,survivin可能作为一个分子标记对淋巴瘤分型具有一定的价值.
目的淋巴瘤的診斷與分型是臨床病理診斷的難點.本研究檢測抗凋亡基因survivin在不同類型淋巴瘤中的錶達,併探討其對淋巴瘤分型的意義.方法用免疫組化法檢測中山大學附屬一院及腫瘤醫院2001年1月～2003年6月219例淋巴瘤、13例淋巴結反應性增生石蠟標本中survivin基因的錶達;同時用RT-PCR檢測K562、HL60、Raji、Jurkat細胞繫和以上病例中18例淋巴瘤及2例淋巴結反應性增生新鮮標本中survivin mRNA的錶達;對不同類型的淋巴瘤survivin蛋白及mRNA錶達的結果進行半定量分析.結果 Survivin蛋白在非霍奇金淋巴瘤(NHL)的瀰漫性大B細胞性淋巴瘤(DLBL)(88.6％,70/79)、伯基特淋巴瘤(BL)(100％,2/2)、淋巴母細胞淋巴瘤(LBL)(92.3％,12/13)中有較高的錶達,而在濾泡性淋巴瘤(FL)(18.2％),粘膜相關性結外邊緣帶B細胞淋巴瘤(MAL-oma)(40.9％)和MZL(marginal zone lymphoma)(33.3％)中錶達較低,且多為弱暘性.高錶達組(DLBL、BL、LBL)與低錶達組(FL、MZL、MALT)之間差異有統計學意義,χ2檢驗,P＜0.01.霍奇金淋巴瘤(HL)中大部分R-S(Reed-Sternberg)細胞錶達survivin蛋白.NHL中survivin mRNA的檢測結果與其蛋白水平的錶達呈正相關(相關繫數r=0.6270,P＜0.01).結論 survivin蛋白及mRNA錶達水平在不同類型淋巴瘤存在著明顯的差異,survivin可能作為一箇分子標記對淋巴瘤分型具有一定的價值.
목적림파류적진단여분형시림상병리진단적난점.본연구검측항조망기인survivin재불동류형림파류중적표체,병탐토기대림파류분형적의의.방법용면역조화법검측중산대학부속일원급종류의원2001년1월～2003년6월219례림파류、13례림파결반응성증생석사표본중survivin기인적표체;동시용RT-PCR검측K562、HL60、Raji、Jurkat세포계화이상병례중18례림파류급2례림파결반응성증생신선표본중survivin mRNA적표체;대불동류형적림파류survivin단백급mRNA표체적결과진행반정량분석.결과 Survivin단백재비곽기금림파류(NHL)적미만성대B세포성림파류(DLBL)(88.6％,70/79)、백기특림파류(BL)(100％,2/2)、림파모세포림파류(LBL)(92.3％,12/13)중유교고적표체,이재려포성림파류(FL)(18.2％),점막상관성결외변연대B세포림파류(MAL-oma)(40.9％)화MZL(marginal zone lymphoma)(33.3％)중표체교저,차다위약양성.고표체조(DLBL、BL、LBL)여저표체조(FL、MZL、MALT)지간차이유통계학의의,χ2검험,P＜0.01.곽기금림파류(HL)중대부분R-S(Reed-Sternberg)세포표체survivin단백.NHL중survivin mRNA적검측결과여기단백수평적표체정정상관(상관계수r=0.6270,P＜0.01).결론 survivin단백급mRNA표체수평재불동류형림파류존재착명현적차이,survivin가능작위일개분자표기대림파류분형구유일정적개치.
Objective: To detect the expression of survivin in subtypes of lymphoma and its value in classifying subtypes of lymphoma. Methods: Paraffin-embedded samples collected from 219 cases of lymphoma and 13 cases of lymph node reactive proliferation from affiliated hospitals of Sun Yat-sen University during 2001-2003 were examined for the expression of survivin by using immunohistochemical staining. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of survivin in K562, HL60, Raji, and Jurkat cell lines. Semi-quantitative assay was used to evaluate the quantity of survivin protein and mRNA expression in subtypes of lymphoma. Results: Protein expression of survivin was high and strong in diffuse large B-cell lymphoma (DLBL) (88.6%, 70/79), Burkitt lymphoma (BL)(100%, 2/2), and lymphoblastic lymphoma (LBL) (92.3%, 12/13), while their expression was always lower and weaker in follicular lymphoma (FL) (18.2%), extranodal marginal zone B-cell lymphoma of mucosaassociated lymphoid tissue (MALT lymphoma) (40.9%) and marginal zone lymphoma (MZL) (33.3%).There was a significant difference between the higher expression group (DLBL, BL and LBL) and lower one (FL, MZL, and MALT) in the expression of survivin (Chi-square test, X2=24.77, P＜0.01). Almost all of Reed-Sternberg cells (R-S cells) in Hodgkin lymphoma (HL) strongly expressed survivin. The protein expression of survivin was positively correlated with mRNA (r=0.6270, P＜0.01). Conclusion: The expression level of survivin mRNA and protein shows significant difference in subtypes of lymphoma. The expression of survivin mRNA might act as a biomarker to classify the subtypes of lymphoma.
