CAJ | 학술논문

选用定位于普通小麦7个部分同源群上的276对微卫星引物对普通小麦中国春和簇毛麦的基因组DNA进行扩增分析,有148对引物可在两个物种间检测到多态性.利用上述显示多态性的引物进一步对7个中国春-簇毛麦二体附加系进行扩增分析,筛选出分别可用来追踪簇毛麦1V至7V染色体的引物wmc49(1BS)、wmc25(2BS)、gdm36(3DS)、gdm145(4AL)、wmc233(5DS)、wmc256(6AL)和gwm344(7BL).此外还发现6DS上的微卫星引物gwm469可以用来追踪簇毛麦的2V染色体;2DS上的微卫星引物gdm107可用来追踪簇毛麦的6V染色体.进一步用涉及不同簇毛麦和小麦背景的小麦-簇毛麦染色体附加系、代换系和易位系进行扩增分析,这些微卫星标记也可用来鉴定簇毛麦的各条染色体.因此,这些簇毛麦染色体特异的微卫星标记可用来追踪普通小麦背景中的簇毛麦染色体.
선용정위우보통소맥7개부분동원군상적276대미위성인물대보통소맥중국춘화족모맥적기인조DNA진행확증분석,유148대인물가재량개물충간검측도다태성.이용상술현시다태성적인물진일보대7개중국춘-족모맥이체부가계진행확증분석,사선출분별가용래추종족모맥1V지7V염색체적인물wmc49(1BS)、wmc25(2BS)、gdm36(3DS)、gdm145(4AL)、wmc233(5DS)、wmc256(6AL)화gwm344(7BL).차외환발현6DS상적미위성인물gwm469가이용래추종족모맥적2V염색체;2DS상적미위성인물gdm107가용래추종족모맥적6V염색체.진일보용섭급불동족모맥화소맥배경적소맥-족모맥염색체부가계、대환계화역위계진행확증분석,저사미위성표기야가용래감정족모맥적각조염색체.인차,저사족모맥염색체특이적미위성표기가용래추종보통소맥배경중적족모맥염색체.
Wheat (Triticum aestivum L.) microsatellite markers were screened for detecting Haynaldia villosa L. chromosomes introduced into wheat background. Two hundred and seventy six primer pairs mapped on 7 homeologous groups of wheat were used to amplify the gDNA of T. aestivum and H. villosa. The results showed that 148 of 276 microsatellite primers amplified polymorphic bands between common wheat cv. Chinese Spring and H. villosa. Primers wmc49 (1BS), wmc25 (2BS), gdm36 (3DS),gdm145 (4AL), wmc233 (5DS), wmc256 (6AL) and gwm344 (7BL) produced a specific polymorphic DNA fragment on chromosome 1V to 7V of H. villosa, respectively. In addition, gwm469 (6DS) detected a specific band on 2V; gdm107 (2DS) amplified a specific band on 6V. These microsatellite markers were effective in identifying individual H. villosa chromosomes in other T. aestivum-H. villosa chromosome addition, substitution and translocation lines involved in different H. villosa accessions and wheat backgrounds. Therefore, these chromosome-specific microsatellite markers could be used as molecular markers for detection of chromosomes of H. villosa in common wheat.