CAJ | 학술논문

采用相同种类卵水诱导的方法对直翅目,蟋蟀科,黄脸油葫芦的受精囊精子的顶体反应过程进行系统观察.发现黄脸油葫芦精子顶体反应可划分为3个阶段,第1阶段,精子质膜膨胀、断裂或丢失;第2阶段,顶体复合体的顶体外层与顶体本体外膜发生融合,囊泡化;第3阶段,顶体复合体大部分脱落,只留有短锥状的顶体位于核前端.据观察,蟋蟀精子质膜不参与囊泡形成,此结果与家蝇及哺乳类的猪、牛、绵羊、猕猴精子的顶体反应结果很相似.经过比较发现卵水对受精囊内精子的诱导率明显高于精巢内,据分析,可能与精子的生理成熟有关,即便受精囊内精子比精巢内精子更趋于成熟.与其他学者的实验结果相比,蟋蟀精子顶体反应率与家蝇的相似,但明显低于其他动物.这可能与动物的授精方式有关.
채용상동충류란수유도적방법대직시목,실천과,황검유호호적수정낭정자적정체반응과정진행계통관찰.발현황검유호호정자정체반응가화분위3개계단,제1계단,정자질막팽창、단렬혹주실;제2계단,정체복합체적정체외층여정체본체외막발생융합,낭포화;제3계단,정체복합체대부분탈락,지류유단추상적정체위우핵전단.거관찰,실천정자질막불삼여낭포형성,차결과여가승급포유류적저、우、면양、미후정자적정체반응결과흔상사.경과비교발현란수대수정낭내정자적유도솔명현고우정소내,거분석,가능여정자적생리성숙유관,즉편수정낭내정자비정소내정자경추우성숙.여기타학자적실험결과상비,실천정자정체반응솔여가승적상사,단명현저우기타동물.저가능여동물적수정방식유관.
The morphological ultrastructure variation during the acrosome reaction of spermatozoa cricket in Teleogryllus emma(Ohmachi et Matsumura), in the spermathecas and testes respectively are investigated with inducement method of same-species egg water solution (EWS). The sperm acrosome complex generally possesses three-layer structures, i. e., extra-acrosome layer,acrosome and perforatorium. The process of the acrosome reaction consists of several stages, i. e., the front of the extra-acrosome layer swelling and acrosome extending forward, the extra-acrosome layer and acrosome outer membrane fusing and vesiculating, as well as the acrosome falling and a short perforatorium remaining on the top of the nucleus.Compared with the acrosome complex structures of the sperm between the spermatheca and thestis, there are haven't any differentence in the structures.