CAJ | 학술논문

以海洋底栖硅藻新月菱形藻MMDL533作为模型污损物种,利用显微镜-图像分析软件、平行平板流动装置测定了其在羟基化玻璃表面及改性玻璃上的附着性质.研究表明,环境温度为20℃时,新月菱形藻MMDL533在羟基化玻璃表面的附着量最大,在1 h的接触时间后达到87±9 /mm~2;同时,其附着量随着接触时间延长而变大,并在3 h后达到一个较为稳定的值(110±15 /mm~2左右);当用水流冲刷后细胞的移除率来表征细胞在特定表面的附着强度时,新月菱形藻MMDL533的附着强度随着表面接触角逐渐增大,并在80°左右达到最大值,然后呈下降趋势.
이해양저서규조신월릉형조MMDL533작위모형오손물충,이용현미경-도상분석연건、평행평판류동장치측정료기재간기화파리표면급개성파리상적부착성질.연구표명,배경온도위20℃시,신월릉형조MMDL533재간기화파리표면적부착량최대,재1 h적접촉시간후체도87±9 /mm~2;동시,기부착량수착접촉시간연장이변대,병재3 h후체도일개교위은정적치(110±15 /mm~2좌우);당용수류충쇄후세포적이제솔래표정세포재특정표면적부착강도시,신월릉형조MMDL533적부착강도수착표면접촉각축점증대,병재80°좌우체도최대치,연후정하강추세.
The settlement and adhesion of marine benthic diatom Nitzschia closterium MMDL533 were studied on the fresh and cleaned glass slides (bearing hydroxyl groups) and a series of the fresh cleaned glass slides treated by silane surface chemistry to produce a self-assembly monolayer (SAM).Upon surface adhesion under different conditions,the diatom attached the surfaces were subjected to shear flow from a parallel plate flow apparatus to assess the strength of surface adhesion.The results showed that the diatom settlement on the surface of the hydroxyl-slides was temperature and time dependent,as expected.Interestingly,however,the strength of surface adhesion was also time dependent.After 7 hr surface attachment,there were clearly signs of splitting of surface attached diatoms,showing the growth under the experimental conditions.It was also found that the surface adhesion of Nitzschia closterium MMDL533 was the strongest at the contact angle around 80,and that away from this region the resistance to detachment by the wall shear stress showed a clear trend of decrease.This trend was clearly different from other diatoms such as Amphora coffeaeformis var.purpusilla,showing that both surface properties and diatom types could affect the surface adhesion.